2026-06-29T02:37:41Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1125972025-07-30T17:36:07Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Žagar, Anamarija author Dajčman, Urban author Megia Palma, Rodrigo Manuel author Simčič, Tatjana author Barroso, Frederico M. author Baškiera, Senka author Carretero, Miguel A. 2024-12-13T09:40:52Z 2024-12-13T09:40:52Z 2024 Žagar, A., Dajčman, U., Megía-Palma, R., Simčič, T., Barroso, F. M., Baškiera, S., & Carretero, M. A. (2024). Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions. Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, 297. https://doi.org/10.1016/J.CBPA.2024.111729 1095-6433 10.1016/j.cbpa.2024.111729 https://hdl.handle.net/20.500.14352/112597 1531-4332 https://doi.org/10.1016/j.cbpa.2024.11172 https://www.sciencedirect.com/science/article/pii/S1095643324001569?via%3Dihub Aerobic respiration is the main energy source for most eukaryotes, and efficient mitochondrial energy transfer greatly influences organismal fitness. To survive environmental changes, cells have evolved to adjust their biochemistry. Thus, measuring energy metabolism at the subcellular level can enhance our understanding of individual performance, population dynamics, and species distribution ranges. We investigated three important metabolic traits at the subcellular level in five lacertid lizard species sampled from different elevations, from sea level up to 2000 m. We examined hemoglobin concentration, two markers of oxidative stress (catalase activity and carbonyl concentration) and maximum rate of metabolic respiration at the subcellular level (potential metabolic activity at the electron transport system). The traits were analysed in laboratory acclimated adult male lizards to investigate the adaptive metabolic responses to the variable environmental conditions at the local sampling sites. Potential metabolic activity at the cellular level was measured at four temperatures – 28 °C, 30 °C, 32 °C and 34 °C – covering the range of preferred body temperatures of the species studied. Hemoglobin content, carbonyl concentration and potential metabolic activity did not differ significantly among species. Interspecific differences were found in the catalase activity, Potential metabolic activity increased with temperature in parallel in all five species. The highest response of the metabolic rate with temperature (Q10) and Arrhenius activation energy (Ea) was recorded in the high-mountain species Iberolacerta monticola. eng Attribution-NonCommercial-NoDerivatives 4.0 International Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditions journal article 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