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oai:docta.ucm.es:20.500.14352/1128562024-12-19T01:03:20Zcom_20.500.14352_14col_20.500.14352_15

The Crosstalk between Cardiac Lipotoxicity and Mitochondrial Oxidative Stress in the Cardiac Alterations in Diet-Induced Obesity in Rats Jiménez González, Sara Marín-Royo, Gema Jurado-López, Raquel Bartolomé Bartolomé Pascual, María Visitación Miranda-Romero, Ana Luaces Méndez, María Islas, Fabian Nieto, María Luisa Martínez-Martínez Martínez Martínez, Ernesto Cachofeiro, Victoria Cachofeiro Ramos, María Victoria The impact of the mitochondria-targeted antioxidant MitoQ was evaluated in the cardiac alterations associated with obesity. Male Wistar rats were fed either a high fat diet (HFD, 35% fat) or a standard diet (CT, 3.5% fat) for 7 weeks and treated with MitoQ (200 µM). The effect of MitoQ (5 nM) in rat cardiac myoblasts treated for 24 h with palmitic acid (PA, 200 µM) was evaluated. MitoQ reduced cardiac oxidative stress and prevented the development of cardiac fibrosis, hypertrophy, myocardial 18-FDG uptake reduction, and mitochondrial lipid remodeling in HFD rats. It also ameliorated cardiac mitochondrial protein level changes observed in HFD: reductions in fumarate hydratase, complex I and II, as well as increases in mitofusin 1 (MFN1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha, and cyclophilin F (cycloF). In vitro, MitoQ prevented oxidative stress and ameliorated alterations in mitochondrial proteins observed in palmitic acid (PA)-stimulated cardiac myoblasts: increases in carnitine palmitoyltransferase 1A, cycloF, and cytochrome C. PA induced phosphorylation of extracellular signal-regulated kinases and nuclear factor-κB p65. Therefore, the data show the beneficial effects of MitoQ in the cardiac damage induced by obesity and suggests a crosstalk between lipotoxicity and mitochondrial oxidative stress in this damage 2024-12-18T08:57:06Z 2024-12-18T08:57:06Z 2020-02-17 journal article Jiménez-González S, Marín-Royo G, Jurado-López R, Bartolomé MV, Romero-Miranda A, Luaces M, Islas F, Nieto ML, Martínez-Martínez E, Cachofeiro V. Crosstalk between Cardiac Lipotoxicity and Mitochondrial Oxidative Stress in the Cardiac Alterations in Diet-Induced Obesity in Rats.Cells. 2020 Feb 17;9(2):451. doi: 10.3390/cells9020451. 2073-4409 10.3390/cells9020451 https://hdl.handle.net/20.500.14352/112856 https://www.mdpi.com/2073-4409/9/2/451 https://pubmed.ncbi.nlm.nih.gov/32079154/ eng PI18/00257 PI15/00742 CIBERCV SAF2016-81063 http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International MDPI