2026-06-28T04:56:19Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1142282025-01-15T00:58:11Zcom_20.500.14352_14col_20.500.14352_15

Santiago, Begoña Galindo Izquierdo, María Rivero, Miguel Brehmer, M. Mateo, I. Pablos Álvarez, José Luis 2025-01-14T11:38:57Z 2025-01-14T11:38:57Z 2002-07-01 Santiago B, Galindo M, Rivero M, Brehmer MT, Mateo I, Pablos JL. The chemoattraction of lymphocytes by rheumatoid arthritis - synovial fluid is not dependent on the chemokine receptor CCR5. Rheumatol Int. 2002 Jul;22(3):107-11. 0172-8172 10.1007/s00296-002-0203-1 https://hdl.handle.net/20.500.14352/114228 1437-160X https://doi.org/10.1007/s00296-002-0203-1 https://link.springer.com/article/10.1007/s00296-002-0203-1 Objective: The objective was to study the potential role of the chemokine receptor CCR5 in the chemoattraction of lymphocytes by rheumatoid arthritis synovial fluid (RA-SF). Methods: The expression of the CCR5 receptor was studied by flow cytometry. Chemotaxis of peripheral blood lymphocytes in response to RA-SF was analyzed on transmigration chambers. Chemotaxis of immortalized lymphocytes from individuals homozygous for the Delta32 deletion of the CCR5 gene (CCR5-/-) was analyzed. The effect of a neutralizing anti-CCR5 antibody on the migration of CCR5+/+ cells was also studied. Results: We confirmed an increase in the proportion of CCR5-expressing lymphocytes in RA-SF and a preferential migration of CCR5+ lymphocytes toward RA-SF in vitro. CCR5-/- lymphocytes showed decreased chemotactic responses to the chemokine MIP-1beta but not to RA-SF. The chemotactic responses of CCR5+/+ lymphocytes to RA-SF were not modified by anti-CCR5 neutralizing antibody. Conclusions: We confirm a preferential accumulation of CCR5-expressing lymphocytes into RA-SF. However, the chemotactic responses of lymphocytes to RA-SF were not dependent on a functional CCR5 receptor, suggesting that CCR5 is a marker of a lymphocyte subset rather than a specific mediator of chemotactic responses to chemokines in RA-SF. eng restricted access The chemoattraction of lymphocytes by rheumatoid arthritis - synovial fluid is not dependent on the chemokine receptor CCR5 journal article