2026-06-29T02:31:49Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1142952025-03-18T13:42:35Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Civera, Alba author Tvorynska, Sofiia author Gamella Carballo, María author Torrente Rodríguez, Rebeca Magnolia author Pedrero Muñoz, María author Galan-Malo, Patricia author Mata, Luis author Sanchez, Lourdes author Barek, Jiri author Pingarrón Carrazón, José Manuel author Campuzano Ruiz, Susana author Perez, Maria D. author 2023 A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng/mL of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg/ g of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique. Alba Civera, Sofiia Tvorynska, Maria Gamella, Rebeca M. Torrente-Rodríguez, María Pedrero, Patricia Galán-Malo, Luis Mata, Lourdes Sánchez, Jiří Barek, José M. Pingarrón, Susana Campuzano, María D. Pérez, First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6, Microchemical Journal, Volume 195, 2023, 109403 10.1016/j.microc.2023.109403 https://hdl.handle.net/20.500.14352/114295 https://www.sciencedirect.com/science/article/pii/S0026265X23010226?via%3Dihub First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6