2026-06-28T20:24:50Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1143172025-03-18T11:50:45Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Linillos Pradillo, Beatriz author Rancán, Lisa author Díaz Ramiro, Elías author Vara Ameigeiras, Elena María author Artíñano, Begoña author Arias Díaz, Javier 2025-01-14T16:27:34Z 2025-01-14T16:27:34Z 2021-04 Linillos-Pradillo B, Rancan L, Ramiro ED, Vara E, Artíñano B, Arias J. Determination of SARS-CoV-2 RNA in different particulate matter size fractions of outdoor air samples in Madrid during the lockdown. Environ Res. 2021 Apr;195:110863. doi: 10.1016/j.envres.2021.110863. Epub 2021 Feb 17. PMID: 33609549; PMCID: PMC7888991. 0013-9351 10.1016/j.envres.2021.110863 https://hdl.handle.net/20.500.14352/114317 https://10.1016/j.envres.2021.110863 Background: Previous studies described the presence of SARS-CoV-2 in outdoor air particulate matter (PM) in urban areas of northern Italy and USA. The city of Madrid was heavily affected by COVID-19 during March-June 2020. Also, this city usually displays high concentrations of PM under several atmospheric situations. This is mandatory to assess the presence of viral RNA in PM, as an indicator of epidemic recurrence. Our study was aimed at investigating the presence of SARS-CoV-2 RNA in outdoor air samples (on PM10, PM2.5 and PM1). Methods: Six samples of PM10, PM2.5 and PM1 were collected between the May 4th and 22nd 2020 in Madrid, on quartz fiber filters by using MCV high volume samplers (30 m3 h-1 flow) with three inlets (Digitel DHA-80) for sampling PM10, PM2.5 and PM1. RNA extraction and amplification was performed according to the protocol recently set by Setti et al.2020c in Italy. Up to three highly specific molecular marker genes (N1, N2, and RP) were used to test the presence of SARS-CoV-2 RNA. Results: After RNA extraction and expression measurements of N1, N2 and RP genes from all the collected filters, no presence of SARS-CoV-2 RNA was observed. Control tests to exclude false positive results were successfully accomplished. Conclusions: No presence of SARS-CoV-2 in quartz fiber filters samplers for PM10, PM2.5 and PM1 fractions was observed in our study carried out in Madrid during the month of May 2020. Nevertheless, the absence of viral genomes could be due to different factors including: limited social interactions and economic activities resulting in reduced circulation of the coronavirus, lower daily PM concentration in outdoor air, as well as to meteorological stability and higher temperature that characterize spring season. Further research should be carried out during winter, in presence of higher viral circulation and daily PM exceedances. eng Attribution-NonCommercial-NoDerivatives 4.0 International Determination of SARS-CoV-2 RNA in different particulate matter size fractions of outdoor air samples in Madrid during the lockdown journal article 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URL https://docta.ucm.es/bitstreams/45eed1e0-a771-432a-a76e-2cd3558aa346/download File MD5 f69a9d1b842d84c5877067be375b1d65 3893222 application/pdf Determinación del ARN del SARS-CoV-2 enpdf URL https://docta.ucm.es/bitstreams/06e5fb27-8745-4611-9355-68a2cf29fc2b/download File MD5 1f286058ce00401dfe595f7dfefb286d 44008 text/plain Determinación del ARN del SARS-CoV-2 enpdf.txt