2026-06-07T17:24:41Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1147252025-01-17T00:57:59Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Torrente Rodríguez, Rebeca Magnolia author Muñoz San Martín, Cristina author Gamella Carballo, María author Pedrero Muñoz, María author Martínez Bosch, Neus author Navarro, Pilar author García de Frutos, Pablo author Pingarrón Carrazón, José Manuel author Campuzano Ruiz, Susana author 2021-06-21 A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL−1) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease. Torrente-Rodríguez, R.M.; Martín, C.M.-S.; Gamella, M.; Pedrero, M.; Martínez-Bosch, N.; Navarro, P.; García de Frutos, P.; Pingarrón, J.M.; Campuzano, S. Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases. Biosensors 2021, 11, 202. https://doi.org/10.3390/bios11060202 10.3390/bios11060202 https://hdl.handle.net/20.500.14352/114725 https://doi.org/10.3390/bios11060202 Electrochemical immunosensing of ST2: A checkpoint target in cancer diseases