2026-06-08T01:07:04Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/1147252025-01-17T00:57:59Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Torrente Rodríguez, Rebeca Magnolia
author
Muñoz San Martín, Cristina
author
Gamella Carballo, María
author
Pedrero Muñoz, María
author
Martínez Bosch, Neus
author
Navarro, Pilar
author
García de Frutos, Pablo
author
Pingarrón Carrazón, José Manuel
author
Campuzano Ruiz, Susana
2025-01-16T14:44:53Z
2025-01-16T14:44:53Z
2021-06-21
Torrente-Rodríguez, R.M.; Martín, C.M.-S.; Gamella, M.; Pedrero, M.; Martínez-Bosch, N.; Navarro, P.; García de Frutos, P.; Pingarrón, J.M.; Campuzano, S. Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases. Biosensors 2021, 11, 202. https://doi.org/10.3390/bios11060202
10.3390/bios11060202
https://hdl.handle.net/20.500.14352/114725
https://doi.org/10.3390/bios11060202
A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL−1) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Electrochemical immunosensing of ST2: A checkpoint target in cancer diseases
journal article
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URL
https://docta.ucm.es/bitstreams/bf01096b-2c89-4960-a507-c1d249729c12/download
File
MD5
6b992c4b13b8e3c2766fa71da5327de0
1116845
application/pdf
Torrente-Rodríguez2021biosensors-11-00202_ST2.pdf