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oai:docta.ucm.es:20.500.14352/1149262025-01-18T01:03:03Zcom_20.500.14352_14col_20.500.14352_15

Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors Vyšniauskas, Aurimas López Duarte, Ismael Duchemin, Nicolas Vu, Thanh-Truc Wu, Yilei Budynina, Ekaterina M. Volkova, Yulia A. Peña Cabrera, Eduardo Ramírez-Ornelas, Diana E. Kuimova, Marina K. Microviscosity is a key parameter controlling the rate of diffusion and reactions on the microscale. One of the most convenient tools for measuring microviscosity is by fluorescent viscosity sensors termed ‘molecular rotors’. BODIPY-based molecular rotors in particular proved extremely useful in combination with fluorescence lifetime imaging microscopy, for providing quantitative viscosity maps of living cells as well as measuring dynamic changes in viscosity over time. In this work, we investigate several new BODIPY-based molecular rotors with the aim of improving on the current viscosity sensing capabilities and understanding how the structure of the fluorophore is related to its function. We demonstrate that due to subtle structural changes, BODIPY-based molecular rotors may become sensitive to temperature and polarity of their environment, as well as to viscosity, and provide a photophysical model explaining the nature of this sensitivity. Our data suggests that a thorough understanding of the photophysics of any new molecular rotor, in environments of different viscosity, temperature and polarity, is a must before moving on to applications in viscosity sensing. 2025-01-17T13:53:09Z 2025-01-17T13:53:09Z 2025-01-17T13:53:09Z 2017 journal article https://hdl.handle.net/20.500.14352/114926 1463-9076 1463-9084 10.1039/c7cp03571c eng Vyšniauskas A, López-Duarte I, Duchemin N, Vu TT, Wu Y, Budynina EM, et al. Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors. Phys Chem Chem Phys [Internet]. 2017 [citado 17 de enero de 2025];19(37):25252-9. Disponible en: https://xlink.rsc.org/?DOI=C7CP03571C http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Royal Society of Chemistry