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oai:docta.ucm.es:20.500.14352/1150142025-03-18T14:50:18Zcom_20.500.14352_14col_20.500.14352_15

Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells Larrosa Pérez, María Del Mar Burgos-Edwards, A Martín-Pérez, L Jiménez-Aspee, F Theoduloz, C Schmeda-Hirschmann, G 615.322 Ribes spp Intestinal inflammation COX-2 iNOS interleukin secretion Medicamentos 32 Ciencias Médicas The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β). The inhibitory effect of non-digested and digested PEEs towards human cyclooxygenase 1 (COX-1) and COX-2, and the gene expression of COX-2 and inducible nitric oxide synthase (iNOS) was also evaluated. The digested PEE from R. punctatum decreased the secretion of IL-8, IL-6, and TNF-α; whereas R. magellanicum reduced IL-6 and TNF-α in the Caco-2 cells (p < 0.05). Both digested extracts significantly down-regulated the mRNA expression of COX-2 and iNOS (p < 0.05). PEEs showed 60% of inhibition towards COXs, with higher inhibition against COX-2. The PEEs from R. punctatum displayed better anti-inflammatory activity in all the experiments. Our results suggest that R. magellanicum and R. punctatum might be useful against intestine inflammatory conditions. Ministerio Economía y Competitividad Programa de Investigación de Excelencia Interdisciplinaria en Química y Bio-orgánica de Recursos Naturales (PIEI-QUIM-BIO) de la Universidad de Talca Depto. de Nutrición y Ciencia de los Alimentos Fac. de Farmacia TRUE pub 2025-01-20T08:41:34Z 2025-01-20T08:41:34Z 2019-08-01 journal article VoR https://hdl.handle.net/20.500.14352/115014 XXXX-XXXX 10.1016/j.jff.2019.06.007 eng Ramón y Cajal (referencia: 2012_11910) AGL2016-77288-R Burgos-Edwards A, Martín-Pérez L, Jiménez-Aspee F, Theoduloz C, Schmeda-Hirschmann G, Larrosa M. Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells: Anti-inflammatory activity of in vitro digested Chilean currants. J Funct Foods. 2019;59:329-36. doi:10.1016/j.jff.2019.06.007 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf Elsevier