2026-06-28T20:12:40Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/1151002025-08-21T12:29:47Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Zubeldia-Brenner, Lautaro
author
Gutiérrez Uzquiza, Álvaro
author
Barrio-Real, Laura
author
Wang, Hongbin
author
Marcelo G. Kazanietz
author
Leskow, Federico Coluccio
2025-01-20T12:19:14Z
2025-01-20T12:19:14Z
2014
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L. et al. β3-Chimaerin, a novel member of the chimaerin Rac-GAP family. Mol Biol Rep 41, 2067–2076 (2014). https://doi.org/10.1007/s11033-014-3055-3
0301-4851
10.1007/s11033-014-3055-3
https://hdl.handle.net/20.500.14352/115100
https://doi.org/10.1007/s11033-014-3055-3
Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
journal article
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