2026-06-08T01:07:52Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1152252025-03-18T13:24:13Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Checa Herráiz, Rocío author Fidalgo, Luis Eusebio author Montoya Matute, Ana author López, Ana María author Barrera, Juan Pedro author Gálvez Esteban, Rosa María author Sánchez de la Nieta, Sara author Marino, Valentina author Sarquis, Juliana author Miró Corrales, Guadalupe 2025-01-20T16:56:38Z 2025-01-20T16:56:38Z 2019-03-26 Checa, R., Fidalgo, L.E., Montoya, A. et al. The role of healthy dog carriers of Babesia microti-like piroplasms. Parasites Vectors 12, 127 (2019). https://doi.org/10.1186/s13071-019-3371-5 1756-3305 10.1186/s13071-019-3371-5 https://hdl.handle.net/20.500.14352/115225 https://doi.org/10.1186/s13071-019-3371-5 Background: While in Europe Babesia canis has been traditionally held responsible for canine piroplasmosis, Babesia microti-like piroplasm (Bml) infection is being ever more observed in dogs, with the first clinical cases reported in northwestern Spain. This study examines the epidemiological role of healthy dogs living in endemic areas of Bml infection in Spain. The data obtained were used to describe the clinical status and map the geographical distribution of Bml infection in healthy dogs in northwestern Spain. Results: Blood samples and ticks were taken from 756 healthy dogs representatively across the whole Galicia region (northwestern Spain): stray (n = 211), hunting dogs (n = 333) and pets (n = 212). Blood samples were tested by microscopy parasite observation, nested PCR-RFLP and sequencing. Piroplasm infection prevalences in healthy dogs from northwestern Spain were 17.1% (129/756) by PCR and 3.4% (26/756) by microscopy observation. The species found by PCR were: 2.2% (17/756) for B. canis and 15.1% (114/756) for Bml. Co-infection with B. canis and Bml was noted in 2 dogs. The higher prevalences detected were Bml in hunting dogs (25.5%; 85/333) and B. canis in stray dogs (6.6%; 14/211). In fox-hunting dogs from any area and dogs from the A Coruña Province, significantly higher prevalences of Bml infection were detected (P < 0.001). Upon physical examination, tick infestation was observed: 130 ticks in 18 hunting and three pet dogs. These were subsequently identified as Rhipicephalus sanguineus (s.l.) (49.2%), Ixodes hexagonus (38.5%), Ixodes ricinus (6.9%) and Dermacentor reticulatus (5.4%). Among the more prevalent ticks infesting healthy carrier dogs were I. hexagonus, followed by D. reticulatus and I. ricinus. Conclusions: Babesia canis and Bml were the only piroplasm species found infecting healthy dogs in Galicia, the prevalence of Bml being higher than of B. canis. Factors correlated with a higher Bml infection risk were being a hunting dog and living in the A Coruña Province. Healthy dogs travelling to other countries could act as carriers and probably contribute to the spread of Bml infection in dogs and wild carnivores throughout Europe. eng Attribution 4.0 International The role of healthy dog carriers of Babesia microti‑like piroplasms journal article 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URL https://docta.ucm.es/bitstreams/95c84217-3fae-4831-ac8c-e54b58b84c0b/download File MD5 726c7eaee096785171518b31deef3214 2160567 application/pdf Checa et al., 2019.pdf URL https://docta.ucm.es/bitstreams/dbb2423f-9915-4feb-9a83-5565c4bbc93e/download File MD5 95179f1e2d4c12d9ae199addd0432d18 58602 text/plain Checa et al., 2019.pdf.txt