2026-06-29T02:40:34Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1153732025-03-18T16:05:40Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Gómez Delgado, Irene author López Pastor, Andrea R. author González Jiménez, Adela author Ramos Acosta, Carlos author Hernández Garate, Yenitzeh author Martínez Micaelo, Neus author Amigó, Núria author Espino Paisán, Laura author Anguita Mandly, Eduardo Luis author Urcelay, Elena 2025-01-21T13:08:36Z 2025-01-21T13:08:36Z 2025-01-10 Gómez-Delgado, I., López-Pastor, A.R., González-Jiménez, A. et al. Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19. Cell Biol Toxicol 41, 27 (2025). https://doi.org/10.1007/s10565-024-09976-0 1573-6822 10.1007/s10565-024-09976-0 https://hdl.handle.net/20.500.14352/115373 https://doi.org/10.1007/s10565-024-09976-0 https://link.springer.com/article/10.1007/s10565-024-09976-0 The underlying mechanisms explaining the differential course of SARS-CoV-2 infection and the potential clinical consequences after COVID-19 resolution have not been fully elucidated. As a dysregulated mitochondrial activity could impair the immune response, we explored long-lasting changes in mitochondrial functionality, circulating cytokine levels, and metabolomic profiles of infected individuals after symptoms resolution, to evaluate whether a complete recovery could be achieved. Results of this pilot study evidenced that different parameters of aerobic respiration in lymphocytes of individuals recuperated from a severe course lagged behind those shown upon mild COVID-19 recovery, in basal conditions and after simulated reinfection, and they also showed altered glycolytic capacity. The severe groups showed trends to enhanced superoxide production in parallel to lower OPA1-S levels. Unbalance of pivotal mitochondrial fusion (MFN2, OPA1) and fission (DRP1, FIS1) proteins was detected, suggesting a disruption in mitochondrial dynamics, as well as a lack of structural integrity in the electron transport chain. In serum, altered cytokine levels of IL-1β, IFN-α2, and IL-27 persisted long after clinical recovery, and growing amounts of the latter after severe infection correlated with lower basal and maximal respiration, ATP production, and glycolytic capacity. Finally, a trend for higher circulating levels of 3-hydroxybutyrate was found in individuals recovered after severe compared to mild course. In summary, long after acute infection, mitochondrial and metabolic changes seem to differ in a situation of full recovery after mild infection versus the one evolving from severe infection. eng Attribution-NonCommercial-NoDerivatives 4.0 International Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19 journal article 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URL https://docta.ucm.es/bitstreams/f78e0ba0-3261-4639-9a7f-1707b16e2c45/download File MD5 c336d4223372e3b3bac548f322bef596 3244206 application/pdf Long‑term mitochondrial and metabolic impairment .pdf URL https://docta.ucm.es/bitstreams/2c7d2993-f76f-489b-9e37-f4eb9261fd48/download File MD5 982f6c91028fadc6655ab5c2c14432d5 77061 text/plain Long‑term mitochondrial and metabolic impairment .pdf.txt