2026-06-28T20:26:15Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1157242025-03-18T11:51:56Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Marco-Martín G author La Rotta Hernández A, author Vázquez de la Torre M author Higaki Y author Zubeldia Ortuño, José Manuel author Baeza ML 2025-01-23T08:54:39Z 2025-01-23T08:54:39Z 2017-08-30 1423-0097 10.1159/000478983 https://hdl.handle.net/20.500.14352/115724 1018-2438. https://doi.org/10.1159/000478983 https://pubmed.ncbi.nlm.nih.gov/19852821/https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-6-44 Background: Anaphylaxis is a severe and potentially lethal allergic reaction whose incidence is increasing. Murine models can elucidate the underlying mechanisms and pave the way for appropriate therapeutic options. However, differences in strains and protocols hamper comparisons of data between researchers. We performed a parallel study of clinical and immune responses with 2 strains of mice, BALB/c and C3H/HeOuJ, in an allergen-induced systemic anaphylaxis protocol. Both strains have been widely used in allergy models, although they have not been compared in an intraperitoneal systemic model. Methods: Groups of 5-week-old female BALB/c and C3H/HeOuJ mice were intraperitoneally sensitized with peanut in the presence of adjuvants. Specific immunoglobulin (sIg) G1, sIgG2a, sIgE, total IgE, histamine release, and specific stimulated splenocyte cytokines, interleukin (IL)-4, IL-5, IL-10, IL-12, IL-13, and interferon (IFN)-γ, were assessed. At week 6, mice were intraperitoneally challenged with peanut. Anaphylaxis was evaluated by recognition of clinical symptoms and changes in body temperature. Results: All peanut-sensitized mice induced sIg and developed anaphylactic symptoms upon challenge. Nonetheless, the C3H/HeOuJ strain demonstrated earlier and persistently higher sIgG1 and sIgG2a production, elevated sIgE, and more severe clinical symptoms and histamine release than the BALB/c strain. In contrast, BALB/c exhibited higher release of IL-4, IL-5, IL-10, IL-13, and IFN-γ. Conclusions: Both models are suitable for studying anaphylaxis. Consequently, they could be used in research on the pathogenesis and therapy of anaphylaxis. However, according to the type of study performed, differences in the specific clinical, humoral, and cellular responses to antigens have to be considered. eng Attribution-NonCommercial-NoDerivatives 4.0 International Differences in the Anaphylactic Response between C3H/HeOuJ and BALB/c Mice journal article 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URL https://docta.ucm.es/bitstreams/ddad2b52-d32b-43e2-a5c4-c1b11affe68f/download File MD5 6000332443e47eba3f7c5f4f15eb56e4 184172 application/pdf Differences in the Anaphylactic Response between C3H_HeOuJ and BALB_c Mice.pdf URL https://docta.ucm.es/bitstreams/a4b18a42-152d-41d2-96b0-943ce07e89f7/download File MD5 7277667abe15dea66bdfef8ac65c35d6 42849 text/plain Differences in the Anaphylactic Response between C3H_HeOuJ and BALB_c Mice.pdf.txt