2026-06-27T12:39:08Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1164092025-01-28T00:58:39Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Garcimartín Álvarez, Alba author Merino Martín, José Joaquín author González Prieto, María Del Pilar author Sánchez Reus, María Isabel author Sánchez Muniz, Francisco José author Bastida Codina, Sara author Benedí González, Juana María 2025-01-27T16:37:36Z 2025-01-27T16:37:36Z 2014 Garcimartín, A., Merino, J.J., González, M.P. et al. Organic silicon protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide effects. BMC Complement Altern Med 14, 384 (2014). https://doi.org/10.1186/1472-6882-14-384 1472-6882 10.1186/1472-6882-14-384 https://hdl.handle.net/20.500.14352/116409 https://doi.org/10.1186/1472-6882-14-384 https://pmc.ncbi.nlm.nih.gov/articles/PMC4203892/ Background: Hydrogen peroxide (H2O2) is a toxic agent that induces oxidative stress and cell death. Silicon (Si) is a biological element involved in limiting aluminium (Al) absorption with possible preventive effects in Alzheimer's disease. However, Si has not yet been associated with other neuroprotective mechanisms. Methods: The present experiments evaluated in the SH-SY5Y human neuroblastoma cell line the possible role of different Si G5 (50-1000 ng/mL) concentrations in preventing cellular death induced by H2O2 (400 μM, 24 hours). Results: Our findings showed that H2O2 promoted cell death in the human SH-SY5Y cell cultures and this could be prevented by Si treatment. The loss in cell viability mediated by H2O2 was due to an apoptotic and necrotic process. Apoptotic death was incurred by regulating caspase-8 activity in the extrinsic pathway. The apoptotic and necrotic cell death induced by H2O2 was almost totally reversed by Si (50-500 ng/mL), indicating that it down-regulates both processes in H2O2 treated cells. Conclusions: According to our data, Si is able to increase SH-SY5Y cell survival throughout partially blocking cellular damage related to oxidative stress through a mechanism that would affect H2O2/ROS elimination. eng Attribution-NonCommercial-NoDerivatives 4.0 International Organic silicon protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide effects journal article 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URL https://docta.ucm.es/bitstreams/38668334-b352-4ec8-849c-9175035106b1/download File MD5 da7ef0451e08903ee803785020312b5a 787244 application/pdf Organic_silicon.pdf