2026-06-29T02:49:16Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1166192025-03-18T13:17:37Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Moyano-Cires Ivanoff, Paula Viviana author García Sánchez, José Manuel author García Lobo, Jimena author Pelayo Alarcón, Adela author Muñoz-Calero, Pilar author Frejo Moya, María Teresa author Anadón Baselga, María José author Naval López, María Victoria author Flores, Andrea author Mirat, Vega Alejandra author Pino Sans, Javier Del author 2021-04-27 Chlorpyrifos (CPF) biocide, is associated with breast cancer. The processes underlying this association have not been elucidated to date. CPF increases MCF-7 and MDA-MB-231 cell proliferation after acute and long-term treatment, partially through KIAA1363 overexpression and aryl-hydrocarbon receptor activation but also through estrogen receptor-alpha activation after 24 h exposure in MCF-7 cells, suggesting other mechanisms may be involved. CPF induces reactive oxygen species (ROS) generation, acetylcholine accumulation, and overexpression of acetylcholinesterase-R/S (AChE-R/S) variants, while it also alters the Wnt/β-catenin pathway, both in vitro and in vivo, in processes different from cancer. These latter mechanisms are also linked to cell proliferation and could mediate this effect induced by CPF. Our results show that CPF (0.01–100 μM), following one-day and fourteen-days treatment, respectively, induced ROS generation and lipid peroxidation, and acetylcholine accumulation due to AChE inhibition, Wnt/β-catenin up- or downregulation depending on the CPF treatment concentration, and AChE-R and AChE-S overexpression, with the latter being mediated through GSK-3β activity alteration. Finally, CPF promoted cell division through ACh and ROS accumulation, AChE-R overexpression, and Wnt/β-catenin signaling disruption. Our results provide novel information on the effect of CPF on human breast cancer cell lines that may help to explain its involvement in breast cancer. Moyano, P., García, J. M., García, J., Pelayo, A., Muñoz-Calero, P., Frejo, M. T., ... & Del Pino, J. (2021). Chlorpyrifos induces cell proliferation in MCF-7 and MDA-MB-231 cells, through cholinergic and Wnt/β-catenin signaling disruption, AChE-R upregulation and oxidative stress generation after single and repeated treatment. Food and Chemical Toxicology, 152, 112241. 0278-6915 10.1016/j.fct.2021.112241 https://hdl.handle.net/20.500.14352/116619 https://doi.org/10.1016/j.fct.2021.112241 Chlorpyrifos induces cell proliferation in MCF-7 and MDA-MB-231 cells, through cholinergic and Wnt/β-catenin signaling disruption, AChE-R upregulation and oxidative stress generation after single and repeated treatment