2026-06-29T01:19:21Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/1173432025-03-18T15:12:14Zcom_20.500.14352_14col_20.500.14352_15
Macrophage-dependent neutrophil recruitment is impaired under conditions of increased intestinal permeability in JAM-A-deficient mice
Luissint, Anny-Claude
Williams, Holly C.
Kim, Wooki
Flemming, Sven
Hilgarth, Roland S.
O'Leary, Monique N.
Denning, Tomothy L.
Nusrat, Asma
Parkos, Charles A.
Azcutia Criado, Verónica
Junctional adhesion molecule-A (JAM-A) is a transmembrane glycoprotein expressed on leukocytes, endothelia, and epithelia that regulates biological processes including barrier function and immune responses. While JAM-A has been reported to facilitate tissue infiltration of leukocytes under inflammatory conditions, the contributions of leukocyte-expressed JAM-A in vivo remain unresolved. We investigated the role of leukocyte-expressed JAM-A in acute peritonitis induced by zymosan, lipopolysaccharide (LPS), or TNFα using mice with selective loss of JAM-A in myelomonocytic cells (LysM-Cre;Jam-afl/fl). Surprisingly, in LysM-Cre;Jam-afl/fl mice, loss of JAM-A did not affect neutrophil (PMN) recruitment into the peritoneum in response to zymosan, LPS, or TNFα although it was significantly reduced in Jam-aKO mice. In parallel, Jam-aKO peritoneal macrophages exhibited diminished CXCL1 chemokine production and decreased activation of NF-kB, whereas those from LysM-Cre;Jam-afl/fl mice were unaffected. Using Villin-Cre;
2025-01-30T14:51:11Z
2025-01-30T14:51:11Z
2025-01-30T14:51:11Z
2019
journal article
https://hdl.handle.net/20.500.14352/117343
XXXX-XXXX
10.1038/s41385-019-0143-7
eng
1R01DK097256
DK59888
DK55679
DK072564
DK061379
DK079392
Luissint, AC., Williams, H.C., Kim, W. et al. Macrophage-dependent neutrophil recruitment is impaired under conditions of increased intestinal permeability in JAM-A-deficient mice. Mucosal Immunol 12, 668–678 (2019). https://doi.org/10.1038/s41385-019-0143-7
http://creativecommons.org/licenses/by-nc-nd/4.0/
open access
Attribution-NonCommercial-NoDerivatives 4.0 International
Springer Nature