2026-06-28T15:19:11Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1179222025-09-02T13:37:19Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author García-Cardoso, Juan author Zamorano-León, José J. author González-Enguita, Carmen author Carbantes-Alarcon, David author Zekri-Nechar, Khaoula author Simón Adiego, Carlos María author Jiménez García, Rodrigo author López De Andrés, Ana Isabel author Cuadrado Corrales, María Natividad author Martínez Martínez, Carlos Hugo 2025-02-07T15:35:05Z 2025-02-07T15:35:05Z 2024 García-Cardoso J, Zamorano-León JJ, González-Enguita C, Simón C, Jiménez-García R, López-de-Andrés A, Cuadrado-Corrales N, Carbantes-Alarcon D, Martínez-Martínez CH, Zekri-Nechar K. Phosphodiesterase-5 Expression in Buccal Mucosa of Patients with Erectile Dysfunction One Year after Radical Prostatectomy. J Pers Med. 2024 Aug 17;14(8):869 10.3390/jpm14080869 https://hdl.handle.net/20.500.14352/117922 2075-4426 https://dx.doi.org/10.3390/jpm14080869 39202060 https://www.mdpi.com/2075-4426/14/8/869 Background: Radical prostatectomy has a high incidence of erectile dysfunction (ED). The aim was to determine if the expression of the nitric oxide synthase-3/soluble guanylate cyclase/phosphodiesterase 5 axis could be detected in buccal mucosa and if it could be differently expressed in patients with and without ED; Methods: Erectile function from 38 subjects subjected to prostatectomy was evaluated using the International Index of Erectile Function-Erectile Function Domain before and one year after surgery. Nitric oxide synthase (NOS3), beta 1-subunit of soluble guanylate cyclase (sGC), phosphodiesterase-5 (PDE-5) expressions, and interleukin-6 and interleukin-10 content were measured in the buccal mucosa. PDE5A rs3806808 gene polymorphism was genotyped; Results: One year after prostatectomy, 15 patients had recovered functional erection, and 23 showed ED. NOS3, beta 1-sGC, interleukin-6, and interleukin-10 expressions were not different between patients with and without ED after radical prostatectomy. Buccal mucosa levels of PDE-5 were higher in patients with ED compared to those who recovered erectile functionality. There were no differences found in the genotype of PDE5A polymorphism; Conclusions: One year after prostatectomy, patients with ED had higher PDE5 levels in their buccal mucosa than patients who had recovered erectile function. Rs3806808 PDE5A gene polymorphism was not associated with increased PDE5 expression in buccal mucosa. eng Attribution 4.0 International Phosphodiesterase-5 Expression in Buccal Mucosa of Patients with Erectile Dysfunction One Year after Radical Prostatectomy journal article 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