2026-08-20T15:39:44Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1187562025-09-16T15:04:20Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Serna Bernaldo, Carlos author Calderón Bernal, Johan Manuel author Torre Fuentes, Laura author García Muñoz, Ángel author Díez Guerrier, Alberto Antoine author Hernández, Marta author Fernández-Garayzábal Fernández, José Francisco author Vela Alonso, Ana Isabel author Cid Vázquez, María Dolores author Álvarez Sánchez, Julio 2025-03-13T14:49:26Z 2025-03-13T14:49:26Z 2025 Serna, C., Calderón Bernal, J. M., Torre-Fuentes, L., García Muñoz, Á., Díez Guerrier, A., Hernández, M., Fernández-Garayzábal, J. F., Vela, A. I., Cid, D., & Alvarez, J. (2025). Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots. The veterinary quarterly, 45(1), 1–15. https://doi.org/10.1080/01652176.2025.2474220 0165-2176 10.1080/01652176.2025.2474220 https://hdl.handle.net/20.500.14352/118756 1875-5941 https://doi.org/10.1080/01652176.2025.2474220 40055923 The emergence of multidrug-resistance (MDR) in Pasteurella multocida, a major contributor to bovine respiratory disease (BRD) is being increasingly reported, often linked to the carriage of antimicrobial resistance genes (ARGs) on integrative and conjugative elements (ICEs). The resistance phenotype for 19 antimicrobials was determined using broth microdilution in 75 Pasteurella multocida isolates from healthy and BRD-affected cattle from five feedlots. The genomes of 32 isolates were sequenced to identify ARG) and mobile genetic elements (MGEs) and assess their genetic diversity. MDR isolates (with phenotypic resistance to aminoglycosides, macrolides, fluoroquinolones and/or tetracyclines) were primarily found among BRD-affected compared to healthy animals. Non-susceptible isolates, belonging to ST79 and ST13, harbored point mutations and four to nine ARGs, including rarely reported mechanisms in Europe (mph(E), msr(E) and aadA31 ARGs and newly described mutations in the gyrA/parC genes). All ARGs were linked to the presence of MGEs including two ICEs, Tn7407 and the novel Tn7809, a prophage and a putative composite transposon. Clonally related isolates were found in different batches from the same feedlot, suggesting maintenance of MDR strains. Our findings demonstrate the diverse genetic basis of AMR in P. multocida from BRD-affected cattle in Spain, emphasizing the role of MGEs in the ARG dissemination. eng Attribution-NonCommercial 4.0 International Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots journal article 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URL https://docta.ucm.es/bitstreams/13faa117-df08-4043-87b6-87d909d3fd6b/download File MD5 3b35fb1689301cd3ca425902a1caf0bc 4291222 application/pdf Integrative and conjugative.pdf URL https://docta.ucm.es/bitstreams/c36e439d-a3fb-4b50-90d7-dda1cbff3376/download File MD5 ec19ce83c8e2c14ad46a61540c11c40c 81930 text/plain Integrative and conjugative.pdf.txt