2026-06-28T20:28:22Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1190282025-03-29T00:59:33Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Esteso, Cristina Milagros author Toledano Díaz, Adolfo author Castaño, Cristina author Higes, Mariano author Martín Hernández, Raquel author López Goya, Agustin author De la Rúa, Pilar author Martínez Madrid, Carmen Belén author Santiago Moreno, Julián author 2025 The present study aimed to evaluate an optimal method to transport and store drone sperm samples, as well as to characterize drone spermatozoa through sperm head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, sperm variables were assessed under different incubation conditions (5 °C, 15 °C, and 37 °C with 5% CO2). Results showed that sperm viability was optimally maintained at 15 °C (p ˂ 0.05). In the second experiment, the supplementation of extender with catalase (200 UI) improved (p ˂ 0.05) the sperm viability and motility during liquid storage at different incubation times. Finally, a morphometric analysis of sperm head was made: length 5.13 µm, width 0.85 µm, area 3.78 µm2, perimeter 15.01 µm, acrosome length 3.50 µm. The variability in sperm head morphometry was calculated by coefficients of variation (CV) within- and between-drones. The CV within-drone was higher than the CV between-drones for all morphometric parameters regardless of hive origin, indicating a high degree of sperm pleomorphism Simple Summary: Environmental factors may produce morphological and morphometric alterations in the honey bee’s sperm. These changes in drone spermatozoa can produce variations in their physical characteristics, which could cause the loss of ability to remain viable within the queen bee’s spermatheca. The storage and conservation of sperm is an effective strategy to protect the genetic diversity of honey bees and contribute to selective breeding programs. The extender choice greatly impacts sperm quality. The use of antioxidants in extenders for preservation could improve the functional semen characteristics. Indeed, the supplementation of extender with catalase (200 UI) improved the sperm viability and motility during liquid storage. The accurately measured dimensions of sperm head allow improving the understanding of reproductive biology and might even be used as indicators of environmental influences. The variability of sperm head morphometry was calculated, revealing a high degree of sperm pleomorphism in drones, suggesting the existence of sub-populations that should be investigated in the future. Esteso, M. C., Toledano-Díaz, A., Castaño, C., Higes, M., Martín-Hernández, R., López-Goya, A., De la Rúa, P., Martínez-Madrid, B., & Santiago-Moreno, J. (2025). Storage Conditions of Sperm Samples and Gametic Characterization by Sperm Head Morphometry in Drones (Apis mellifera). Animals, 15(5), 672. https://doi.org/10.3390/ani15050672 10.3390/ani15050672 https://hdl.handle.net/20.500.14352/119028 2076-2615 https://doi.org/10.3390/ani15050672 Storage Conditions of Sperm Samples and Gametic Characterization by Sperm Head Morphometry in Drones (Apis mellifera)