2026-06-28T20:31:58Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/119482024-04-16T17:32:17Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Sánchez Salcedo, Sandra
author
Vallet Regí, María Dulce Nombre
author
Allaf Shanin, Sophia
author
Zinck, Jeffrey I.
author
Glackin, Carlotta A.
2023-06-17T12:26:02Z
2023-06-17T12:26:02Z
2018-01-07
1385-8947
10.1016/j.cej.2017.12.116
https://hdl.handle.net/20.500.14352/11948
https://www.elsevier.com/
http://www.ucm.es/valletregigroup
Mesoporous silica nanoparticles (MSNPs) have many potential applications in biomedical fields. However, when MSNPs are exposed to plasma, protein adsorption leads to opsonization and decreases blood circulation time. A new multifunctional nanodevice based on polyethylenimine (PEI) coated core-shell Fe3O4@SiO2 MSNPs with a zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) surface was designed to minimize unspecific protein adhesion. Particle size measurements demonstrated an excellent non-fouling capacity in solutions containing Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) plasma proteins. The system was used in this study to co-deliver two different cargos: siRNA and daunorubicin. Anti-TWIST siRNA plays critical role in modulating knockdown of TWIST and sensitizing cells to chemotherapeutics such as daunorubicin for ovarian cancer therapy. The drug was released in response to externally controlled oscillating magnetic fields (OMF).
siRNA (siGFP) silenced expression of green fluorescence protein (GFP) in Ovcar8 cancer cells, demonstrating the incorporation of core shell MSNPs into cells and siGFP delivery. The synergistic effect of the co-release of anti-TWIST-siRNA loaded in the PEI and daunorubicin loaded in NPs’ pores caused increased cytotoxicity in Ovcar8 of up to 50% from both zwitteronic and non-zwitteronic NPs. The system is the first example of silencing by anti-TWITS-siRNA/daunorubicin co-delivered using zwitterionic core-shell nanoparticles with low-fouling adsorption. This engineered multifunctional approach may provide therapeutic potential for the treatment of currently incurable ovarian cáncer.
eng
Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
journal article
URL
https://docta.ucm.es/bitstreams/aa1aa9ac-2dce-451a-8a2d-a724e884c832/download
File
MD5
5d8c3d29f8fe9a67c7e375e53164d875
3518953
application/pdf
33. Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarium cancer therapy.pdf
URL
https://docta.ucm.es/bitstreams/2c1ef749-9a82-41b4-9288-6fd6c2717357/download
File
MD5
5bca5425b289ac07670e183c84334b33
55479
text/plain
33. Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarium cancer therapy.pdf.txt