2026-06-01T07:32:52Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1200272025-05-13T23:53:01Zcom_20.500.14352_14col_20.500.14352_15

A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo Li, Shaoguang Ferrer-Ruiz, Andrés Dai, Jun Ramos-Soriano, Javier Du, Xuewei Zhu, Man Zhang, Wanxue Wang, Yuanyuan Herranz Astudillo, María Ángeles Jing, Le Zhang, Zishuo Li, Hui Xia, Fan Martín León, Nazario 547 Biosensor Aptamer PH-independent Redox Electron donor Tetrathiafulvalene Química orgánica (Química) 2306 Química Orgánica Electronic supplementary information (ESI) available. See https://doi.org/10.1039/d2sc02021a The development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments (e.g. subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor p-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both in vitro and in the bladders of living rats. Spanish Ministry of Science and Innovation (MICIN) National Natural Science Foundation of China National Key Research and Development Program of China NSFC-ISF Research Grant Program Zhejiang Provincial Natural Science Foundation of China Hubei Provincial Natural Science Foundation of China Depto. de Química Orgánica Fac. de Ciencias Químicas TRUE pub 2025-05-13T10:17:11Z 2025-05-13T10:17:11Z 2022-06-27 journal article VoR https://hdl.handle.net/20.500.14352/120027 XXXX-XXXX 10.1039/D2SC02021A eng PID2020-114653RB-I00, PID2020-115120GB-I00, RED2018-102815-T and Centro de Excelencia Severo Ochoa SEV-2016-0686 22090050, 21874121, and 22122410 2021YFA1200403 and 2018YFE0206900 Grant No. 22161142020 2020CFA037 Grant No. LD21B050001 Li, S. Ferrer-Ruiz, A. Dai, J. Ramos-Soriano, J. Du, X. Zhu, M. Zhang, W. Wang, Y. Herranz, M.i. Jing, L. Zhang, Z. Li, H. Xia, F. Martín, N. A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo. Chem. Sci., 2022, 13, 8813-8820. Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf Royal Society of Chemistry