2026-06-29T16:41:56Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1217852025-06-25T00:13:53Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Valdivieso González, David author Sacristán Fernández, Miguel Ángel author Natale, Paolo author Orgaz Martín, Belén author Lillo, Pilar M. author Almendro Vedia, Víctor Galileo author López Montero, Iván author 2025 Energy homeostasis in cells relies on the rotary motion of ATP synthase. The spinning movement is also assumed to impact the mechanical properties of the surrounding lipids, leading to emergent effects such as non-equilibrium membrane fluctuations or protein curvature sorting in model systems. Here, we demonstrate that ATP synthase rotation reduces lateral pressure by decreasing lipid packing in both artificial and bacterial membranes. Using micropipette aspiration and fluorescence lifetime imaging microscopy, we find that the rotation of ATP synthase lowers the membrane tension of giant unilamellar vesicles, making the membranes more flexible. Fluorescent probes sensitive to lipid packing confirm that ATP synthase lowers membrane surface pressure during rotation. In vivo experiments in Bacillus subtilis further reveal that stimulation of ATP synthase rotation with specific drugs decreases the lateral pressure of the plasma membrane. These findings suggest that ATP synthase plays a significant role in modulating the mechanical properties of native membranes, potentially driving biological processes linked to membrane dynamics and protein self-assembly. Valdivieso González, D., Sacristán, M. A., Natale, P., Orgaz, B., Lillo, M. P., Almendro-Vedia, V., & López-Montero, I. (2025). Elastic remodeling of model and cell membranes by rotating ATP synthase. Cell Reports Physical Science, 6(5), 102567. https://doi.org/10.1016/j.xcrp.2025.102567 10.1016/j.xcrp.2025.102567 https://hdl.handle.net/20.500.14352/121785 2666-3864 https://doi.org/10.1016/j.xcrp.2025.102567 Elastic remodeling of model and cell membranes by rotating ATP synthase