2026-06-08T13:38:16Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1219002025-07-16T14:03:47Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Valencia, Inés author Pastor-Martínez, Andrea author Decouty-Pérez, Céline author Lopez-Rodriguez, Ana Belen author Álvarez-Rubal, María author Ramos Alonso, Eva author Calzaferri, Francesco author Zamorano-Fernández, Jorge author Giner-García, Javier author Palpán-Flores, Alexis J author Rodríguez-Domínguez, Víctor author Rodríguez de Cía, Javier author Hernández-García, Borja J author Romero Martínez, Manuel Alejandro author de Los Ríos, Cristóbal author Egea, Javier author 2025 Background and purpose: Traumatic brain injury (TBI) is considered to be a leading cause of mortality and disability worldwide. After TBI, innate immunity is rapidly activated in response to damage-associated molecular patterns, such as ATP release, recognised by P2X7 receptors. The P2X7-NLRP3 inflammasome axis has been identified as one of the main players in neuroinflammation. This study aimed to validate P2X7 receptors as therapeutic target for traumatic brain injury. Experimental approach: P2X7 receptors were studied by genetic and pharmacological approaches. Six non-nucleotide purine derivatives were evaluated as P2X7 antagonists. Compounds that prevented LPS + ATP-induced IL-1β release from primary glial cultures were investigated in the closed-head injury TBI model in vivo in male mice. Finally, we evaluated soluble (s)P2X7 receptor plasmatic levels in a cohort of TBI patients. Key results: P2rx7−/− mice showed an exaggerated inflammatory response 24 h post-TBI compared to control mice. However, animals treated with the selective P2X7 antagonist JNJ-47965567 (30 mg kg−1 i.p.) 30 min post-TBI showed improved neurological and inflammatory parameters. The purine derivative ITH15004 was the most potent compound reducing IL-1β production in vitro. When administered in vivo 30 min post-TBI, ITH15004 (1 mg kg−1 i.p.) improved both neuro-behavioural and inflammatory markers at 24 h. In TBI patients, we showed a tendency towards increase in circulating sP2X7 receptor levels at 24 and 72 h post-TBI. Conclusions and implications: These results highlight the importance of P2X7 receptors in the acute phase of TBI and present ITH15004 as a promising pharmacological tool to counteract P2X7 receptor-dependent neuroinflammation in vivo. Valencia, I., Pastor-Martínez, A., Decouty-Pérez, C., Lopez-Rodriguez, A. B., Álvarez-Rubal, M., Ramos, E., Calzaferri, F., Zamorano-Fernández, J., Giner-García, J., Palpán-Flores, A. J., Rodríguez-Domínguez, V., Rodríguez de Cía, J., Hernández-García, B. J., Romero, A., de los Ríos, C., & Egea, J. (2025). Pharmacological evaluation of non-nucleotide purine derivatives as P2X7 antagonists for the treatment of neuroinflammation in traumatic brain injury. British Journal of Pharmacology, 1–17. https://doi.org/10.1111/bph.70108 0007-1188 10.1111/bph.70108 https://hdl.handle.net/20.500.14352/121900 1476-5381 https://doi.org/10.1111/bph.70108 40533073 Pharmacological evaluation of non-nucleotide purine derivatives as P2X7 antagonists for the treatment of neuroinflammation in traumatic brain injury.