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oai:docta.ucm.es:20.500.14352/1219352025-06-27T23:54:42Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Muñoz Picos, Mercedes author López-Oliva Muñoz, María Elvira author Rodríguez Prados, Claudia author Martínez Sainz, María Del Pilar author Sánchez Pina, Ana Alejandra author Climent Flórez, Belén author Benedito Castellote, Sara author García Sacristán, Albino author Rivera De Los Arcos, Luis author Hernández Rodríguez, Medardo Vicente author Prieto Ocejo, Dolores author Sáenz-Medina, Javier author 2020 Oxidative stress-associated endothelial dysfunction is a key pathogenic factor underlying the microvascular complications of metabolic disease. NADPH oxidase (Nox) is a major source of oxidative stress in diabetic nephropathy and chronic kidney disease, despite Nox4 and Nox2 have been identified as relevant sources of vasodilator endothelial HO.The present study was sought to investigate the role of Nox enzymes in renal vascular oxidative stress and endothelial dysfunction in a rat model of genetic obesity. Endothelial function was assessed in intrarenal arteries of obese Zucker rats (OZR) and their counterparts lean Zucker rats (LZR) mounted in microvascular myographs, and superoxide (O) and HO production were measured. Impaired endothelium-dependent relaxations to acetylcholine (ACh) were associated to augmented O generation, but neither ROS scavengers nor the Nox inhibitor apocynin significantly improved these relaxant responses in renal arteries of OZR. Whereas NO contribution to endothelial relaxations was blunted, catalase-sensitive non-NO non-prostanoid relaxations were enhanced in obese rats. Interestingly, NADPH-dependent O production was augmented while NADPH-dependent HO generation was reduced, and cytosolic and mitochondrial SOD were up-regulated in kidney of obese rats. Nox4 was down-regulated in renal arteries and Nox4-dependent HO generation and endothelial relaxation were reduced in OZR. Up-regulation of both Nox2 and Nox1 was associated with augmented O production but reduced HO generation and blunted endothelial Nox2-derived HO-mediated in obese rats. Moreover, increased Nox1-derived O contributed to renal endothelial dysfunction in OZR. In summary, the current data support a main role for Nox1-derived O in kidney vascular oxidative stress and renal endothelial dysfunction in obesity, while reduced endothelial Nox4 expression associated to decreased HO generation and HO-mediated vasodilatation might hinder Nox4 protective renal effects thus contributing to kidney injury. This suggests that effective therapies to counteract oxidative stress and prevent microvascular complications must identify the specific Nox subunits involved in metabolic disease. Muñoz M, López-Oliva ME, Rodríguez C, Martínez MP, Sáenz-Medina J, Sánchez A, et al. Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity. Redox Biology 2020;28:101330. https://doi.org/10.1016/j.redox.2019.101330. 10.1016/j.redox.2019.101330 https://hdl.handle.net/20.500.14352/121935 https://doi.org/10.1016/j.redox.2019.101330 31563085 Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity