2026-06-29T08:22:15Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/123362023-08-28T13:52:56Zcom_20.500.14352_14col_20.500.14352_15

DNA extraction and amplification from Pinaceae dry wood Méndez-Cea, Belén Cobo Simón, Irene Pérez González, Ana García García, Isabel Linares, Juan Carlos Gallego Rodríguez, Francisco Javier 581.15 575 582.47 Dry wood DNA isolation CTAB 18S Abies pinsapo Cedrus atlantica Genética 2409 Genética Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments. Depto. de Genética, Fisiología y Microbiología Fac. de Ciencias Biológicas TRUE pub 2023-06-17T12:30:34Z 2023-06-17T12:30:34Z 2019 journal article https://hdl.handle.net/20.500.14352/12336 2509-8934 10.2478/sg-2019-0010 spa Atribución-NoComercial-SinDerivadas 3.0 España https://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access application/pdf Sciendo