2026-06-28T20:22:24Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1235212025-08-29T23:48:20Zcom_20.500.14352_14col_20.500.14352_15

Unlocking the potential of NH2-MIL-101 (Fe) nanoMOF for advanced electrochemical immunosensing in chronic wound diagnostics Pérez Ginés, Víctor Valero-Calvo, David Torrente Rodríguez, Rebeca Magnolia Pedrero Muñoz, María García Alonso, Francisco J. Pingarrón Carrazón, José Manuel Campuzano Ruiz, Susana Escosura Muniz, Alfredo de la In this work we take advantage, for the first time, of the porous and functional structure of a nanoscale metal organic framework (nanoMOF), specifically NH2-MIL-101 (Fe) nanoMOF, for its use as advantageous enzyme-carrier nanotag in immunoassays. In particular, the porous structure of the nanoMOF allows accommodating a high loading of enzyme molecules, which, in addition, are protected from degradation inside the pores providing a high stability. This property together with the ability of the nanoMOF to link antibodies through its functional groups make it a very robust and powerful label. Both the enhanced sensitivity, compared with the conventional use of enzyme-labelled antibodies, and the high long-term stability are demonstrated. Such nanotag also offers advantages related to ease of modification and cost effectiveness. The detection of myeloperoxidase (MPO), a key biomarker for chronic wound monitoring, is proposed to demonstrate the applicability of this approach. A sandwich-type immunoassay using magnetic beads (MBs)-based platforms and subsequent amperometric detection allows the determination of MPO in the clinical range (5 – 100 ng mL−1), with a limit of detection of 1.4 ng mL−1, and good reproducibility (RSD(n = 10) = 7.1 %). The developed bio-strategy also exhibits high selectivity against potential interferents and a good stability of the nanotag for at least 2 months. Moreover, the developed method was successfully implemented to determine MPO in simulated chronic wound matrices, demonstrating its feasibility in a real scenario. 2025-08-29T09:24:30Z 2025-08-29T09:24:30Z 2025 journal article Víctor Pérez-Ginés, David Valero-Calvo, Rebeca M. Torrente-Rodríguez, María Pedrero, Francisco J. García-Alonso, José M. Pingarrón, Susana Campuzano, Alfredo de la Escosura-Muñiz, Unlocking the potential of NH2-MIL-101 (Fe) nanoMOF for advanced electrochemical immunosensing in chronic wound diagnostics, Sensors and Actuators B: Chemical, Volume 432, 2025, 137460, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2025.137460. 10.1016/j.snb.2025.137460 https://hdl.handle.net/20.500.14352/123521 https://doi.org/10.1016/j.snb.2025.137460 https://www.sciencedirect.com/science/article/pii/S0925400525002357?via%3Dihub eng PID2020-115204RB-I00 RED2022-134120-T info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-136351OB-I00/ES/BIOSENSORES ELECTROQUIMICOS EMPODERADOS PARA TRAZAR NUEVAS HOJAS DE RUTA HACIA LA MEDICINA PERSONALIZADA DEL CANCER, EL ALZHEIMER Y LAS ENFERMEDADES INFECCIOSAS VIRALES/ open access Elsevier