2026-06-24T06:32:36Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1237392025-09-05T23:54:30Zcom_20.500.14352_14col_20.500.14352_22
Docta Complutense advisor Escudero García-Calderón, José Antonio author Trigo da Roza, Filipa Moutinho 2025-09-05T11:22:30Z 2025-09-05T11:22:30Z 2025-09-05 https://hdl.handle.net/20.500.14352/123739 Integrons are genetic elements that capture, rearrange, and disseminateintegron cassettes (IC), including those responsible for antimicrobial resistance (AR).These genetic platforms have significantly contributed to the rise and global spread ofmultidrug-resistant pathogens by recruiting more than 170 different AR genes. However, despite their importance, detecting integron cassettes remains challenging. Conventional approaches, such as PCR, often introduce biases, while more advanced techniques, like deep sequencing, are not yet practical for routine use in laboratories due to their cost and complexity...Los integrones son elementos genéticos capaces de captar, reorganizar y diseminar cassettes de integrones (IC), incluyendo ICs que confieren resistencia aantimicrobianos. Estas plataformas genéticas han contribuido significativamente al aumento y dispersión global de patógenos multirresistentes al reclutar más de 170 genes de resistencia frente a la mayoría de las familias de antibióticos. Sin embargo, a pesar de su importancia, la detección de cassettes de integrón sigue siendo un desafío. Los métodos convencionales, como la PCR, suelen introducir sesgos, mientras que las técnicas más avanzadas, como la secuenciación masiva, aún no están establecidas en el uso rutinario de los laboratorios de diagnóstico debido a su coste y complejidad... eng Chassis de captura de cassettes de integron Integron Cassette capture chassis doctoral thesis 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URL https://docta.ucm.es/bitstreams/3e1a7751-eb81-4295-905d-5793d4df1548/download File MD5 ec0e16b47e65c76a90510c2c90959841 25528951 application/pdf T45479.pdf