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Balas, Delia Fernández Moreira, Esteban Campa, Adela G. de la 2025-10-15T12:05:24Z 2025-10-15T12:05:24Z 1998-06-01 Balas D, Fernández-Moreira E, De La Campa AG. 1998. Molecular Characterization of the Gene Encoding the DNA Gyrase A Subunit of Streptococcus pneumoniae. J Bacteriol 180:. https://doi.org/10.1128/jb.180.11.2854-2861.1998 0021-9193 10.1128/JB.180.11.2854-2861.1998 https://hdl.handle.net/20.500.14352/124943 1098-5530 https://doi.org/10.1128/jb.180.11.2854-2861.1998 https://journals.asm.org/doi/10.1128/jb.180.11.2854-2861.1998?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identi®ed in the promoter region of gyrA. The bend center was mapped and located between the 35 and 10regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended 10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed. eng restricted access Molecular Characterization of the Gene Encoding the DNA Gyrase A Subunit of Streptococcus pneumoniae journal article