2026-06-29T01:34:28Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/125152024-04-09T13:07:12Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Arribas Blázquez, Marina author Olivos Ore, Luis Alcides author Barahona Gomáriz, María Victoria author Sánchez de la Muela, Mercedes author Solar, Virginia author Jiménez, Esperanza author Gualix Sánchez, Francisco Javier author McIntosh, J. Michael author Ferrer-Montiel, Antonio author Miras Portugal, María Teresa author Rodríguez Artalejo, Antonio author 2019-01-03 We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic constriction injury of the sciatic nerve. In slices of the adrenal gland from neuropathic animals, we have evidenced increased cholinergic innervation and spontaneous synaptic activity at the splanchnic nerve–chromaffin cell junction. Likewise, adrenomedullary chromaffin cells displayed enlarged acetylcholine-evoked currents with greater sensitivity to α-conotoxin RgIA, a selective blocker of α9 subunit-containing nicotinic acetylcholine receptors, as well as increased exocytosis triggered by voltage-activated Ca2+ entry. Altogether, these adaptations are expected to facilitate catecholamine output into the bloodstream. Last, but most intriguing, functional and immunohistochemical data indicate that P2X3 and P2X7 purinergic receptors and transient receptor potential vanilloid-1 (TRPV1) channels are overexpressed in chromaffin cells from neuropathic animals. These latter observations are reminiscent of molecular changes characteristic of peripheral sensitization of nociceptors following the lesion of a peripheral nerve, and suggest that similar phenomena can occur in other tissues, potentially contributing to behavioral manifestations of neuropathic pain. 1422-0067 10.3390/ijms20010155 https://hdl.handle.net/20.500.14352/12515 https://doi.org/10.3390/ijms20010155 https://www.mdpi.com/1422-0067/20/1/155 Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain