2026-06-29T02:10:17Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1260012025-11-13T00:47:22Zcom_20.500.14352_14col_20.500.14352_15

A high magnesium concentration in citrate dialysate prevents oxidative stress and damage in human monocytes "in vitro" Vida, Carmen Carracedo Añón, Julia María De Sequera Ortiz, Patricia Bodega, Guillermo Pérez García, Rafael Alique, Matilde Ramírez, Rafael Background. The use of dialysis fluids (DFs) during haemodialysis has been associated with increased oxidative stress and reduced serum magnesium (Mg) levels, contributing to chronic inflammation. Since the role of Mg in modulating immune function and reducing oxidative stress has been demonstrated, the aim of this study was to characterize in vitro whether increasing the Mg concentration in DFs could protect immune cells from oxidative stress and damage. Methods. The effect of citrate [citrate dialysis fluid (CDF), 1 mM] or acetate [acetate dialysis fluid (ADF), 3 mM] dialysates with low (0.5 mM; routinely used) or high (1 mM, 1.25 mM and 2 mM) Mg concentrations was assessed in THP-1 human monocytes. The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and oxidized/reduced (GSSG/GSH) glutathione were quantified under basal and inflammatory conditions (stimulation with lipopolysaccharide, LPS). Results. The increase of Mg in CDF resulted in a significant reduction of ROS production under basal and inflammatory conditions (extremely marked in 2 mM Mg; P < 0.001). These effects were not observed in ADF. Interestingly, in a dose-dependent manner, high Mg doses in CDF reduced oxidative stress in monocytes under both basal and inflammatory conditions. In fact, 2 mM Mg significantly decreased the levels of GSH, GSSG and MDA and the GSSG/GSH ratio in relation to 0.5 mM Mg. Conclusions. CDF produces lower oxidative stress than ADF. The increase of Mg content in DFs, especially in CDF, could have a positive and protective effect in reducing oxidative stress and damage in immune cells, especially under inflammatory conditions. 2025-11-12T08:22:02Z 2025-11-12T08:22:02Z 2025-11-12T08:22:02Z 2020-08-30 journal article https://hdl.handle.net/20.500.14352/126001 2048-8505 10.1093/ckj/sfaa131 2048-8513 eng info:eu-repo/grantAgreement/Instituto de Salud Carlos III/European Commission/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI17%2F01029/Envejecimiento prematuro en pacientes con HQIHUPHGDGUHQDOFUyQLFD avanzada: Un modelo para establecer biomarcadores del riesgo cardiovascular info:eu-repo/grantAgreement/Instituto de Salud Carlos III/European Commission/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI19%2F00240/Vesículas extracelulares como biomarcadores pronósticos de la enfermedad cardiovascular asociada a enfermedad renal crónica info:eu-repo/grantAgreement/Santander/Universidad Complutense de Madrid//PR41%2F17-20964 info:eu-repo/grantAgreement/Universidad Alcalá de Henares//UAH-GP2018-4 info:eu-repo/grantAgreement/Universidad Alcalá de Henares// CCG2018/BIO-010 Vida, C., Carracedo, J., de Sequera, P., Bodega, G., Pérez, R., Alique, M., & Ramírez, R. (2021). A high magnesium concentration in citrate dialysate prevents oxidative stress and damage in human monocytes in vitro. Clinical Kidney Journal, 14(5), 1403-1411. https://doi.org/10.1093/CKJ/SFAA131 http://creativecommons.org/licenses/by-nc/4.0/ open access Attribution-NonCommercial 4.0 International Oxford University Press