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oai:docta.ucm.es:20.500.14352/1260032025-11-13T00:59:04Zcom_20.500.14352_14col_20.500.14352_15

Investigating the Presence of Selenoneine, Ergothioneine, and Selenium-Containing Biomolecules in Fish and Fish-Derived Commercial Products Fernández Bautista, Tamara Gómez Gómez, Beatriz Gracia Lor, Emma Pérez Corona, María Teresa Madrid Albarrán, María Yolanda 543 Selenoneine Ergothioneine Selenium biomolecules Fish-derived products HPLC-ESI-MS/MS HPLC-ICP-MS Ciencias 23 Química Two potent antioxidants, selenoneine and ergothioneine, have been determined simultaneously for the first time in the muscle of highly consumed fish and in fish-derived products. The extraction methods were optimized, and their subsequent analysis was performed by HPLC-ESI-MS/MS. Best extraction efficiency (106 ± 3 and 104 ± 5%) was attained by performing a simple aqueous extraction containing 100 mmol L−1 ammonium acetate or ammonium formate as extractants. Selenoneine and ergothioneine were simultaneously detected in tuna (0.306 ± 0.006 and 3.5 ± 0.2 μg g−1) and swordfish (0.14 ± 0.02 and 1.1 ± 0.4 μg g−1) muscle tissues, while in fish roe and in farmed salmon, only ergothioneine was detected (with contents ranging 0.03−3.3 μg g−1). Moreover, the presence of their respective methylated forms was also considered. Additionally, Se-containing biomolecule profiles from water-soluble proteins were obtained by SEC-ICP-MS analysis. Results revealed similar profiles between fish and fish roe, whereas for ultraprocessed fish products, Se seems to be mostly associated with low-molecular-weight biomolecules. The results included in this work may contribute to a better understanding of the relations between selenoneine and ergothioneine in fishery products as well as their fate and potential modifications of Se-containing biomolecule profiles during fish processing. Unión Europea Comunidad de Madrid Universidad Complutense de Madrid Depto. de Química Analítica Fac. de Ciencias Químicas TRUE pub 2025-11-12T09:00:53Z 2025-11-12T09:00:53Z 2024-11 journal article VoR https://hdl.handle.net/20.500.14352/126003 XXXX-XXXX 10.1021/acs.jafc.4c06111 eng PID2020−114714RB-I00 PID2023−148425NB-I00 2018/BAA-4393 PR65/19− 22432 101007962 Fernández-Bautista, Tamara, et al. «Investigating the Presence of Selenoneine, Ergothioneine, and Selenium-Containing Biomolecules in Fish and Fish-Derived Commercial Products». Journal of Agricultural and Food Chemistry, vol. 72, n.o 47, noviembre de 2024, pp. 26155-64. DOI.org (Crossref), https://doi.org/10.1021/acs.jafc.4c06111. restricted access application/pdf American Chemical Society