2026-06-07T13:54:14Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1260772025-11-15T00:57:34Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Largo-de la Torre, Andrea author Sánchez Sánchez, Roberto author Diezma Díaz, Carlos author Ferré Pérez, Ignacio author Regidor-Cerrillo, Javier author Ortega Mora, Luis Miguel 2025-11-13T14:03:15Z 2025-11-13T14:03:15Z 2025 Largo-de la Torre, A., Sánchez-Sánchez, R., Diezma-Díaz, C., Ferre, I., Regidor-Cerrillo, J., & Ortega-Mora, L. M. (2025). An inactivated vaccine against acquired Toxoplasma gondii infection in pigs as a tool to minimize the zoonotic transmission risk. Veterinary research, 56(1), 206. https://doi.org/10.1186/s13567-025-01645-2 0928-4249 10.1186/s13567-025-01645-2 https://hdl.handle.net/20.500.14352/126077 1297-9716 https://doi.org/10.1186/s13567-025-01645-2 41168831 https://pubmed.ncbi.nlm.nih.gov/41168831/ Toxoplasma gondii, a zoonotic protozoan parasite, represents a major threat to public health and food safety worldwide, particularly through the consumption of undercooked or raw pork containing tissue cysts. Controlling T. gondii infection in pigs is therefore critical to reduce zoonotic transmission. This study evaluated a novel inactivated vaccine formulated with antigens from the Type III TgPigSp1 isolate, comprising both tachyzoite and bradyzoite stages, and adjuvanted with Quil-A. Safety, immunogenicity, and efficacy were assessed first in a murine model as proof of concept and subsequently in piglets challenged with 1000 oocysts of the heterologous Type II-PRU TgShSp1 isolate. The vaccine showed no systemic adverse effects in either model, and an absence of local reactions was observed in piglets. In mice, vaccination elicited strong parasite-specific IgG responses and significantly reduced the parasite burden in brain and muscle tissues by more than 80%. In piglets, robust humoral and cellular immune responses were induced, as evidenced by elevated IgG and IFN-γ levels after the booster vaccination. Following challenge, the parasite load was reduced by ≥ 95% in target muscles, and viable T. gondii cysts were undetectable in at least 50% of vaccinated animals, as confirmed by bioassay. Molecular detection methods further supported the significant reduction in the parasite DNA content. These results indicate that this inactivated multistage vaccine represents a safe, immunogenic, and effective strategy to reduce tissue cyst formation and offer a promising tool within a One Health approach to improve food safety and reduce the burden of toxoplasmosis in both animal and human populations. eng An inactivated vaccine against acquired Toxoplasma gondii infection in pigs as a tool to minimize the zoonotic transmission risk journal article 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URL https://docta.ucm.es/bitstreams/f600f720-bfcd-4253-ac41-e47702aa2314/download File MD5 b2d23f449105814e8d86005af3dde9a9 5129188 application/pdf An inactivated vaccine.pdf