2026-06-29T23:31:55Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1262302025-11-20T01:03:47Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Arribas Antón, Víctor author Monteoliva Díaz, Lucía author Hernáez Sánchez, María Luisa author Gil , Concha author Molero Martín-Portugués, María Gloria author 2024 Abstract: Candida albicans Prn1 is a protein with an unknown function similar to mammalian Pirin. It also has orthologues in other pathogenic fungi, but not in Saccharomyces cerevisiae. Prn1 highly increases its abundance in response to H2O2 treatment; thus, to study its involvement in the oxidative stress response, a C. albicans prn1D mutant and the corresponding wild-type strain SN250 have been studied. Under H2O2 treatment, Prn1 absence led to a higher level of reactive oxygen species (ROS) and a lower survival rate, with a higher percentage of death by apoptosis, confirming its relevant role in oxidative detoxication. The quantitative differential proteomics studies of both strains in the presence and absence of H2O2 indicated a lower increase in proteins with oxidoreductase activity after the treatment in the prn1D strain, as well as an increase in proteasome-activating proteins, corroborated by in vivo measurements of proteasome activity, with respect to the wild type. In addition, remarkable differences in the abundance of some transcription factors were observed between mutant and wild-type strains, e.g., Mnl1 or Nrg1, an Mnl1 antagonist. orf19.4850, a protein orthologue to S. cerevisiae Cub1, has shown its involvement in the response to H2O2 and in proteasome function when Prn1 is highly expressed in the wild type. Arribas, V.; Monteoliva, L.; Hernáez, M.L.; Gil, C.; Molero, G. Unravelling the Role of Candida albicans Prn1 in the Oxidative Stress Response through a Proteomics Approach. Antioxidants 2024, 13, 527. https://doi.org/10.3390/ antiox13050527 10.3390/antiox13050527 https://hdl.handle.net/20.500.14352/126230 https://doi.org/10.3390/antiox13050527 Unravelling the Role of Candida albicans Prn1 in the Oxidative Stress Response through a Proteomics Approach