2026-06-28T20:20:27Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1265052025-12-19T15:01:36Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Jones, Spencer G. author Gil Martí, Beatriz author Sacristán Horcajada, Eva author Edison, Abigail C. author Butler, Emily F. author Miandashti, Neda author Roselli, Camilla author Turiégano, Enrique author Boto, Tamara author Kramer, Jamie M. author Martín Castro, Francisco Antonio author 2025-10 Long-term memory (LTM) requires transcription and translation of new proteins, yet the transcriptional control of memory remains poorly understood. Here, we performed a transcriptome time-course during LTM formation in Drosophila melanogaster exposed to courtship conditioning. We identified a mushroom body-specific transcriptional memory trace that becomes activated during memory consolidation. Using scRNAseq of CREB-activated cells we were able to detect a persistent transcriptional response in MB neurons after LTM consolidation and retrieval. As a proof of causality, we conducted a loss-of-function screen for genes comprising the transcriptional memory trace, finding 16 positive hits whose disruption impaired LTM. Among them, we identified two neuron activity-regulated genes, Hr38 and sr, which encode transcription factors that are activated by courtship LTM training, required for LTM, and bind to many genes comprising the transcriptional memory trace. Overall, we further define the transcriptional response to LTM and identify transcription factors that may help shape it. Jones, S.G., Gil-Martí, B., Sacristán-Horcajada, E. et al. A memory transcriptome time course reveals essential long-term memory transcription factors. Nat Commun 16, 9320 (2025). https://doi.org/10.1038/s41467-025-64379-x 2041-1723 10.1038/s41467-025-64379-x https://hdl.handle.net/20.500.14352/126505 https://doi.org/10.1038/s41467-025-64379-x https://www.nature.com/articles/s41467-025-64379-x A memory transcriptome time course reveals essential long-term memory transcription factors