2026-06-29T03:12:45Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1273682025-11-29T00:58:16Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Muñoz Picos, Mercedes author López-Oliva Muñoz, María Elvira author Pinilla, Estéfano author Martínez Sainz, María Del Pilar author Sánchez Pina, Ana Alejandra author Rodríguez Prados, Claudia author García Sacristán, Albino author Hernández Rodríguez, Medardo Vicente author Rivera De Los Arcos, Luis author Prieto Ocejo, Dolores 2025-11-28T16:13:19Z 2025-11-28T16:13:19Z 2017 Muñoz, M., López-Oliva, M. E., Pinilla, E., Martínez, M. P., Sánchez, A., Rodríguez, C., García-Sacristán, A., Hernández, M., Rivera, L., & Prieto, D. (2017). CYP epoxygenase-derived H2O2 is involved in the endothelium-derived hyperpolarization (EDH) and relaxation of intrarenal arteries. Free radical biology & medicine, 106, 168–183. https://doi.org/10.1016/j.freeradbiomed.2017.02.031 0891-5849 10.1016/j.freeradbiomed.2017.02.031 https://hdl.handle.net/20.500.14352/127368 1873-4596 https://doi.org/10.1016/j.freeradbiomed.2017.02.031 28212823 https://pubmed.ncbi.nlm.nih.gov/28212823/ Reactive oxygen species (ROS) like hydrogen peroxide (H2O2) are involved in the in endothelium-derived hyperpolarization (EDH)-type relaxant responses of coronary and mesenteric arterioles. The role of ROS in kidney vascular function has mainly been investigated in the context of harmful ROS generation associated to kidney disease. The present study was sought to investigate whether H2O2 is involved in the endothelium-dependent relaxations of intrarenal arteries as well the possible endothelial sources of ROS generation involved in these responses. Under conditions of cyclooxygenase (COX) and nitric oxide (NO) synthase inhibition, acetylcholine (ACh) induced relaxations and stimulated H2O2 release that were reduced by catalase and by the glutathione peroxidase (GPx) mimetic ebselen in rat renal interlobar arteries, suggesting the involvement of H2O2 in the endothelium-dependent responses. ACh relaxations were also blunted by the CYP2C inhibitor sulfaphenazole and by the NADPH oxidase inhibitor apocynin. Acetylcholine stimulated both superoxide (O2•-) and H2O2 production that were reduced by sulfaphenazole and apocynin. Expression of the antioxidant enzyme CuZnSOD and of the H2O2 reducing enzymes catalase and GPx-1 was found in both intrarenal arteries and renal cortex. On the other hand, exogenous H2O2 relaxed renal arteries by decreasing vascular smooth muscle (VSM) intracellular calcium concentration [Ca2+]i and markedly enhanced endothelial KCa currents in freshly isolated renal endothelial cells. CYP2C11 and CYP2C23 epoxygenases were highly expressed in interlobar renal arteries and renal cortex, respectively, and were co-localized with eNOS in renal endothelial cells. These results demonstrate that H2O2 is involved in the EDH-type relaxant responses of renal arteries and that CYP 2C epoxygenases are physiologically relevant endothelial sources of vasodilator H2O2 in the kidney. eng CYP epoxygenase-derived H2O2 is involved in the endothelium-derived hyperpolarization (EDH) and relaxation of intrarenal arteries journal article 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URL https://docta.ucm.es/bitstreams/545a9fde-6d85-42b7-aec3-c30ebe982cbb/download File MD5 f3da7c63651e8112c7209149863e107c 2596389 application/pdf CYP epoxygenase-derived.pdf