2026-06-27T10:19:06Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/127612025-08-28T12:43:51Zcom_20.500.14352_14col_20.500.14352_15

Vicario De La Torre, Marta Caballo González, María Vico Ruiz, Eva Morales Fernández, Laura Arriola Villalobos, Pedro Heras Polo, Beatriz De Las Benítez Del Castillo Sánchez, José Manuel Guzmán Navarro, Manuel Millar, Thomas Herrero Vanrell, María Del Rocío Molina Martínez, Irene Teresa 2023-06-17T12:40:43Z 2023-06-17T12:40:43Z 2018 Vicario-de-la-Torre M, Caballo-González M, Vico E, Morales-Fernández L, Arriola-Villalobos P, De Las Heras B, et al. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers 2018;10:425. https://doi.org/10.3390/polym10040425. 2073-4360 10.3390/polym10040425 https://hdl.handle.net/20.500.14352/12761 https://doi.org/10.3390/polym10040425 Dry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4°C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 +/- 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 +/- 1.7 mOsm, with a surface tension of 36.5 +/- 0.4 mN/m and viscosity of 3.05 +/- 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute. eng https://creativecommons.org/licenses/by/4.0/ open access ATTRIBUTION 4.0 INTERNATIONAL Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film journal article