2026-06-28T20:16:18Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1294922025-12-20T01:04:22Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author García Benzaquén, Nerea author Hernández, Marta author Santamaría-Palacios, Jorge author Martínez Alares, Irene author Navarro Gómez, Alejandro author Muñoz-Chimeno, Milagros author Escobar, Franco author Fongaro, Gislaine author Yeramian, Nadine author Trząskowska, Monika author Avellón, Ana author Eiros, José María author Domínguez Rodríguez, Lucas José author Valero, Antonio author Goyache Goñi, Joaquín author Rodríguez-Lázaro, David 2025-12-19T18:21:29Z 2025-12-19T18:21:29Z 2026 García, N., Hernández, M., Santamaría-Palacios, J., Martínez, I., Navarro, A., Muñoz-Chimeno, M., Escobar, F., Fongaro, G., Yeramian, N., Trząskowska, M., Avellón, A., Eiros, J. M., Domínguez, L., Valero, A., Goyache, J., & Rodríguez-Lázaro, D. (2026). Hepatitis E virus in pigs at the moment of slaughter in Spain, 2015 and 2017. Food Microbiology, 136, 105011. https://doi.org/10.1016/j.fm.2025.105011 0740-0020 10.1016/j.fm.2025.105011 https://hdl.handle.net/20.500.14352/129492 1095-9998 https://doi.org/10.1016/j.fm.2025.105011 We investigated the presence of hepatitis E virus (HEV) in pigs at the moment of slaughter in Spain in years 2015 and 2017. A total of 1786 caecal content, liver, and serum samples from animals at slaughterhouses were tested by reverse transcription real-time quantitative PCR (RT-qPCR) and anti-HEV antibodies were evaluated in 623 serum samples by an ELISA test. The overall seroprevalence obtained was 70.9 %. A total of 398 RT-qPCR positive samples were identified in caecal content (26.8 %; 156/583), serum (21.8 %; 136/623) and liver (18.3 %; 106/580). A total of 32 RT-qPCR positive samples were genotyped; 3f (84.4 %) and the 3c (9.4 %) being the most prevalent subgenotypes. This is the first report on detection of HEV in pigs at the moment of slaughter with a Spain nation-wide representation. The data show a large high seroprevalence (70.9 %) in pigs, while the presence of the virus (HEV RNA) was significantly lower. HEV RNA detection varied markedly between matrices, with caecal samples showing higher positivity (30–50 %) than serum (5–25 %); both simple and interaction GEE models confirmed strong effects of sample type and its interaction with year on prevalence estimates. However, the percentage of positive liver samples (18.3 %) and the concurrence between the HEV 3 subtypes identified (3f, 3m and 3c) and those identified in human patients in Spain, underscores the possibility of foodborne zoonosis. It can represent a real risk for consumers if pork products are not cooked adequately. A holistic One-Health approach, including a better understanding of HEV prevalence in the swine population, would allow implementation of control measures in the meat chain to mitigate the main transmission routes for humans. eng Attribution-NonCommercial-NoDerivatives 4.0 International Hepatitis E virus in pigs at the moment of slaughter in Spain, 2015 and 2017 journal article 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URL https://docta.ucm.es/bitstreams/9cbc7774-621b-4ef6-a795-be2023f68a35/download File MD5 3492f785adee77242503dbb6a13c6ea8 2739093 application/pdf Hepatitis E virus.pdf