2026-06-28T15:18:14Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1297442026-01-10T01:14:15Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Andreu-Ballester, Juan Carlos author Hurtado-Marcos, Carolina author García-Ballesteros, Carlos author Pérez-Griera, Jaime author Ollero, Dolores author Jiménez, Ana author Gil-Borrás, Rafael author Llombart-Cussac, Antonio author López-Chuliá, Francisca author Izquierdo Ruiz, Fernando author Cuéllar Del Hoyo, María Del Carmen author 2025-03-28 Background: A deficiency of γδ T cells has been described in Crohn's disease (CD). Aim: To analyze the gene expression of interleukin 7 (IL-7) and its receptors in the tissues of patients with CD. Methods: We studied the peripheral blood of 80 patients with CD, comparing them with a group of 80 healthy subjects. The number and apoptosis of αβ and γδ T cells in peripheral blood and the proportion of αβ and γδ T cells in the intestinal tissues of patients with CD (n = 25) were studied. The gene and protein expression of IL-7, IL-2 receptor subunit γ [cluster of differentiation 132 (CD132)], receptor α (CD127), and caspase-3 in tissues was analyzed by quantitative PCR. Serum IL-7 levels were also analyzed. Results: In patients with CD, a decreased number of γδ T cells and an increase in the apoptosis of CD56+ αβ and γδ T cells in peripheral blood was observed (P < 0.0001 and P < 0.01) respectively, and there was an inverse correlation among T subsets and their apoptosis. In addition, IL-7 gene expression and IL-7 protein in the tissues of these patients were increased. The titers of caspase-3 in tissues were low vs control group (P > 0.01). The percentage of CD8+γδ T cells decreased in tissues (P < 0.01), and was directly related to IL-7 levels in peripheral blood. The expression of IL-2 receptor subunit γ (CD132) was greatly decreased in the tissues of patients with CD (P < 0.05). Conclusion: There may be a cause-effect relationship between the lower gene expression of the IL-2 receptor subunit γ (CD132) in tissues of patients with CD and γδ T cells immunodeficiency. Andreu-Ballester JC, Hurtado-Marcos C, García-Ballesteros C, et al. Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn’s disease. World J Gastroenterol 2025;31. https://doi.org/10.3748/wjg.v31.i12.97120 1007-9327 10.3748/wjg.v31.i12.97120 https://hdl.handle.net/20.500.14352/129744 https://www.wjgnet.com/1007-9327/full/v31/i12/97120.htm Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn’s disease