2026-06-29T02:46:25Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1303792026-01-16T01:18:00Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Saka, Habeeb Kayode author García-Soto, Silvia author Dabo, Nasir Tukur author López Chavarrías, Vicente author Muhammad, Bashir author Ugarte Ruiz, María author Álvarez Sánchez, Julio author 2020 The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presented a ESBLs phenotype, with a significantly higher proportion in isolates from females compared with males (P-value = 0.024). blaCTX-M 73.3% and blaTEM 73.3% were the predominant resistance genes in the ESBLs positive E. coli (each detected in 22/30 isolates, of which 14 harboured both). In addition, 1/30 harboured blaCTX-M + blaTEM + blaSHV genes simultaneously. This study demonstrates the presence of ESBLs E. coli isolates in clinically affected children in Kano, and demonstrates the circulation of blaCTX-M and blaTEM associated with those phenotypes. Enactment of laws on prudent antibiotic use is urgently needed in Kano Saka, H. K., García-Soto, S., Dabo, N. T., Lopez-Chavarrias, V., Muhammad, B., Ugarte-Ruiz, M., & Alvarez, J. (2020). Molecular detection of extended spectrum β-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria. PloS one, 15(12), e0243130. https://doi.org/10.1371/journal.pone.0243130 1932-6203 10.1371/journal.pone.0243130 https://hdl.handle.net/20.500.14352/130379 https://doi.org/ 10.1371/journal.pone.0243130 33270734 Molecular detection of extended spectrum β-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria