2026-06-08T01:18:05Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1304792026-01-17T00:49:30Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Bentancourt Rossoli, Judith V. author Campero, Lucía María author Moré, Gastón author Soto-Cabrera, Agustina author Moore, Dadín P. author Morrell, Eleonora L. author Scioli, María V. author Basso, Walter author Hecker, Yanina Paola author Scioscia, Nathalia P. author 2026 Rodents serve as reservoir hosts for many parasites, including the closely related intracellular protozoan parasites Toxoplasma gondii and Neospora caninum, which are regarded as major causes of abortion in ruminants. This study investigated T. gondii and N. caninum infections in synanthropic (Muridae) and wild (Cricetidae) rodents from rural areas in the Pampas Region, Argentina, using histological, serological, and molecular methods. We also genetically characterized T. gondii isolates. A total of 356 rodents were captured, comprising both murid (Mus musculus, Rattus norvegicus, R. rattus) and cricetid (Oxymycterus rufus, Necromys spp., Akodon azarae, Oligoryzomys flavescens, Calomys musculinus, C. laucha) species. Histological examination did not reveal tissue cysts of these parasites. However, antibodies for T. gondii and N. caninum were detected in 9.8 % (35/356) and 1.7 % (6/356) of the samples using the indirect immunofluorescent antibody test (IFAT). T. gondii DNA was detected by qPCR in brain samples from two of the 41 seropositive rodents. Multilocus PCR-RFLP revealed atypical T. gondii genotypes in both samples. N. caninum DNA was not detected by PCR in any sample. This study provides the first serological and molecular evidence of T. gondii and N. caninum in Cricetidae rodents from Argentina, including the first genotyping data. The detection of T. gondii DNA exclusively in wild rodents highlights the role of wildlife in parasite transmission. Bentancourt Rossoli, J. V., Campero, L. M., Moré, G., Soto-Cabrera, A., Moore, D. P., Morrell, E. L., Scioli, M. V., Basso, W., Hecker, Y. P., & Scioscia, N. P. (2025). Toxoplasma gondii and Neospora caninum in naturally infected synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Parasitology international, 112, 103204. Advance online publication. https://doi.org/10.1016/j.parint.2025.103204 10.1016/j.parint.2025.103204 https://hdl.handle.net/20.500.14352/130479 1383-5769 https://doi.org/10.1016/j.parint.2025.103204 41349635 Toxoplasma gondii and Neospora caninum in naturally infected synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina