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oai:docta.ucm.es:20.500.14352/130622024-07-08T14:55:09Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Sijilmassi, Ouafa author López Alonso, José Manuel author Barrio Asensio, María Del Carmen author Río Sevilla, Aurora Del 2023-06-17T13:19:23Z 2023-06-17T13:19:23Z 2019-01 Sijilmassi, O., López Alonso, J. M., Barrio Asensio, M. C. et al. «Alteration of Lens and Retina Textures from Mice Embryos with Folic Acid Deficiency: Image Processing Analysis». Graefe’s Archive for Clinical and Experimental Ophthalmology, vol. 257, n.o 1, enero de 2019, pp. 111-23. DOI.org (Crossref), https://doi.org/10.1007/s00417-018-4176-5. 0721-832X 10.1007/s00417-018-4176-5 https://hdl.handle.net/20.500.14352/13062 https://doi.org/10.1007/s00417-018-4176-5 https://link.springer.com/article/10.1007/s00417-018-4176-5 Purpose: Folic acid (FA) is an essential vitamin for embryonic development. It plays particularly a critical role in RNA, DNA and protein synthesis. On the other hand, the collagen IV and laminin-1 are important proteins during embryonic development. This study was done to find if FA deficiency at a short and a long term in mothers could alter the tissue texture of retina and lens of the progeny. Methods: Collagen IV and laminin-1 were localized by immunohistochemistry in the lens and retina of the FA-deficient embryos. To carry out the image processing, texture segmentation was performed through canny edge detection and Fourier transform (FT). We defined a parameter, the grain size, to describe the texture of the lens and retina. A bootstrap method to estimate the distribution and confidence intervals of the mean, standard deviation, skewness and kurtosis of the grain size has been developed. Results: Analysis through image processing using Matlab showed changes in the grain size between control- and FA-deficient groups in both studied molecules. Measures of texture based on FT exhibited changes in the directionality and arrangements of type IV collagen and laminin-1. Conclusions: Changes introduced by FA deficiency were visible in the short term (2 weeks) and evident in the long term (8 weeks) in both grain size and orientation of fibre structures in the tissues analysed (lens and retina). This is the first work devoted to study the effect of FA deficit in the texture of eye tissues using image processing techniques. eng Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis journal article URL https://docta.ucm.es/bitstreams/7fc8f890-b6d0-4aa2-9507-3a4e293e857d/download File MD5 f4f16810c8c85f58d30135ff3f5d6c0a 850388 application/pdf Ouafa Sijilmassi_Corrected Manuscript-Alteration.pdf URL https://docta.ucm.es/bitstreams/50e0e846-d9fc-43a9-9c33-1c2e87752c4a/download File MD5 c51e29e6f1d61d239d1c0663b6929d05 42070 text/plain Ouafa Sijilmassi_Corrected Manuscript-Alteration.pdf.txt