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Osteostatin potentiates the bioactivity of mesoporous glass scaffolds containing Zn2+ ions in human mesenchymal stem cell cultures Heras, Clara Sánchez Salcedo, Sandra Lozano Borregón, Daniel Peña, Juan Esbrit, Pablo Vallet Regí, María Dulce Nombre Salinas Sánchez, Antonio Jesús 546 615.46 Mesoporous bioactive glasses ZnO Osteostatin Human mesenchymal cells Materiales Química inorgánica (Farmacia) 3312 Tecnología de Materiales RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo) ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo) RESEARCHER ID B-5081-2017 (Daniel Lozano Borregón) ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí) RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez) ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez) There is an urgent need of biosynthetic bone grafts with enhanced osteogenic capacity. In this study, we describe the design of hierarchical meso-macroporous 3D-scaffolds based on mesoporous bioactive glasses (MBGs), enriched with the peptide osteostatin and Zn2+ ions, and their osteogenic effect on human mesenchymal stem cells (hMSCs) as a preclinical strategy in bone regeneration. The MBG compositions investigated were 80%SiO2– 15%CaO–5%P2O5 (in mol-%) Blank (BL), and two analogous glasses containing 4% ZnO (4ZN) and 5% ZnO (5ZN). By using additive fabrication techniques, scaffolds exhibiting hierarchical porosity: mesopores (around 4 nm), macropores (1-600 m) and big channels ( 1000 m), were prepared. These MBG scaffolds with or without osteostatin were evaluated in cell cultures of hMSCs. Zinc promoted hMSCs colonization (both the surface and inside) of MBG scaffolds. Moreover, Zn2+ ions and osteostatin together, but not independently, in the scaffolds were found to induce the osteoblast differentiation genes runt related transcription factor-2 (RUNX2) and alkaline phosphatase (ALP) in hMSCs after 7 d of culture in the absence of an osteogenic differentiation-promoting medium. These results add credence to the combined use of zinc and osteostatin as an effective strategy for bone regeneration applications. Unión Europea. H2020 Ministerio de Economía, Comercio y Empresa (España) Instituto de Salud Carlos III Depto. de Química en Ciencias Farmacéuticas Fac. de Farmacia TRUE inpress 2023-06-17T13:20:44Z 2023-06-17T13:20:44Z 2019-03-16 journal article https://hdl.handle.net/20.500.14352/13171 1742-7061 10.1016/j.actbio.2019.03.033 eng VERDI (694160) MAT2015-64831-R PI15/00978 Heras, C., Sánchez Salcedo, S., Lozano Borregón, D. et al. «Osteostatin Potentiates the Bioactivity of Mesoporous Glass Scaffolds Containing Zn2+ Ions in Human Mesenchymal Stem Cells». Acta Biomaterialia, vol. 89, abril de 2019, pp. 359-71. DOI.org (Crossref), https://doi.org/10.1016/j.actbio.2019.03.033. open access application/pdf Elsevier