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oai:docta.ucm.es:20.500.14352/1318182026-02-07T00:47:14Zcom_20.500.14352_14col_20.500.14352_15

Improving flap survival by transplantation of a VEGF-secreting endothelised scaffold during distal pedicle flap creation Lasso Vázquez, José María Del Río, Marcela García, María Martínez Calleja V, Nava, Paola Muñoz-Fernández MA, Pérez Cano, Rosa 617-089.844 Adenovirus; VEGF; Gene therapy; Angiogenesis; Flaps Cirugía 32 Ciencias Médicas The benefits of a VEGF-secreting endothelised scaffold on the survival of distal flaps with early pedicle flap division were evaluated in a rabbit model. We bioengineered a scaffold based on a fibrin matrix in which endothelial cells, genetically modified with a VEGF-encoding adenoviral vector were embedded. We distributed subjects in four groups. Control:Eight rabbits conditioned with endothelial cells embedded in a fibrin scaffold. The pedicle was divided after a period of 5 days; and eight rabbits conditioned with endothelial cells embedded in a fibrin scaffold but in this group the pedicle was divided after a period of 2 days. Treatment:Eight rabbits conditioned with VEGF-secreting endothelial cells embedded in a fibrin scaffold; the pedicle was divided after a period of 5 days. And eight rabbits conditioned with VEGF-secreting endothelial cells but the pedicle was divided after a period of 2 days. The percentages of survival were 95.62+/-4.95% for the VEGF-producing scaffold vs. 51.25+/-45.88% for a non-VEGF secreting scaffold when sectioning the pedicle after 5 days. The percentage of survival of flaps in the group grafted with the VEGF-secreting scaffold was 55.62% compared to 2.5% in the group receiving the non-VEGF scaffold when the pedicle was sectioned after 2 days. The CD 31 immunostaining studies showed a dramatic increase in angiogenesis in the flaps conditioned by the VEGF-producing scaffolds. These data revealed that endothelised VEGF-secreting scaffold during flap creation could be a suitable approach in reconstructive surgery. Fundacio´n para la Investigacio´n Sanitaria (FIS) del Ministerio de Sanidad y Consumo (PI052258) (JML) Red Tema´tica Cooperativa de investigacio´n en Gene´tica (RIG C03/07) of FIS Plan Nacional de Salud SAF-2004-06778 (MAMF). Depto. de Cirugía Fac. de Medicina TRUE pub 2026-02-06T12:39:12Z 2026-02-06T12:39:12Z 2007 journal article VoR https://hdl.handle.net/20.500.14352/131818 1748-6815 10.1016/j.bjps.2006.04.002 eng Lasso, J. M., Del Río, M., García, M., Martínez Calleja, V., Nava, P., Muñoz-Fernández, M. A., & Pérez Cano, R. (2007). Improving flap survival by transplantation of a VEGF-secreting endothelised scaffold during distal pedicle flap creation. Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 60(3), 279–286. https://doi.org/10.1016/j.bjps.2006.04.002 restricted access application/pdf Elsevier