2026-06-28T10:37:04Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1328832026-02-26T08:15:53Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Marín-Vicente, Consuelo author Guerrero Valero, Marta author Nielsen, Michael L. author Savitski, Mikhail M. author Gómez-Fernández, Juan Carmelo author Zubarev, Roman A. author Corbalán-García, Senena 2026-02-23T10:58:14Z 2026-02-23T10:58:14Z 2010 Marín-Vicente C, Guerrero-Valero M, Nielsen ML, et al. ATP Enhances Neuronal Differentiation of PC12 Cells by Activating PKCα Interactions with Cytoskeletal Proteins. J Proteome Res 2011;10:529–40. https://doi.org/10.1021/pr100742r 10.1021/pr100742r https://hdl.handle.net/20.500.14352/132883 https://doi.org/10.1021/pr100742r http://europepmc.org/abstract/med/20973479 PKCα is a key mediator of the neuronal differentiation controlled by NGF and ATP. However, its downstream signaling pathways remain to be elucidated. To identify the signaling partners of PKCα, we analyzed proteins coimmunoprecipitated with this enzyme in PC12 cells differentiated with NGF and ATP and compared them with those obtained with NGF alone or growing media. Mass spectrometry analysis (LC−MS/MS) identified plectin, peripherin, filamin A, fascin, and β-actin as potential interacting proteins. The colocalization of PKCα and its interacting proteins increased when PC12 cells were differentiated with NGF and ATP. Peripherin and plectin organization and the cortical remodeling of β-actin were dramatically affected when PKCα was down-regulated, suggesting that all three proteins might be functional targets of ATP-dependent PKCα signaling. Taken together, these data demonstrate that PKCα is essential for controlling the neuronal development induced by NGF and ATP and interacts with the cytoskeletal components at two levels: assembly of the intermediate filament peripherin and organization of cortical actin. eng ATP enhances neuronal differentiation of PC12 cells by activating PKCα interactions with cytoskeletal proteins. journal article 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URL https://docta.ucm.es/bitstreams/2f341c0d-5c58-4dd9-a1cc-f3fcb4cc6b2d/download File MD5 76bed2f4a6dceb3fcef05fcf92bba589 3885254 application/pdf ATP Enhances Neuronal Differentiation of PC12 Cells by Activating PKCα Interactions with Cytoskeletal Proteins_final.pdf