2026-06-28T20:20:29Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1332572026-02-26T01:18:26Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Lorente Leal, Víctor author Damien Farrel author Álvarez Sánchez, Julio author Romero Martínez, Beatriz author Juan Ferré, Lucía De author Stephen V. Gordon 2026-02-25T16:04:02Z 2026-02-25T16:04:02Z 2021 Lorente-Leal V, Farrell D, Romero B, Álvarez J, Juan Ld and Gordon SV (2021) Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization. Front. Vet. Sci. 8:780018. doi: 10.3389/fvets.2021.780018 10.3389/fvets.2021.780018 https://hdl.handle.net/20.500.14352/133257 2297-1769 https://doi.org/10.3389/fvets.2021.780018 34970617 Whole genome sequencing (WGS) and allied variant calling pipelines are a valuable tool for the control and eradication of infectious diseases, since they allow the assessment of the genetic relatedness of strains of animal pathogens. In the context of the control of tuberculosis (TB) in livestock, mainly caused by Mycobacterium bovis, these tools offer a high-resolution alternative to traditional molecular methods in the study of herd breakdown events. However, despite the increased use and efforts in the standardization of WGS methods in human tuberculosis around the world, the application of these WGS-enabled approaches to control TB in livestock is still in early development. Our study pursued an initial evaluation of the performance and agreement of four publicly available pipelines for the analysis of M. bovis WGS data (vSNP, SNiPgenie, BovTB, and MTBseq) on a set of simulated Illumina reads generated from a real-world setting with high TB prevalence in cattle and wildlife in the Republic of Ireland. The overall performance of the evaluated pipelines was high, with recall and precision rates above 99% once repeat-rich and problematic regions were removed from the analyses. In addition, when the same filters were applied, distances between inferred phylogenetic trees were similar and pairwise comparison revealed that most of the differences were due to the positioning of polytomies. Hence, under the studied conditions, all pipelines offer similar performance for variant calling to underpin real-world studies of M. bovis transmission dynamics. eng Attribution 4.0 International Performance and Agreement Between WGS Variant Calling Pipelines Used for Bovine Tuberculosis Control: Toward International Standardization journal article 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URL https://docta.ucm.es/bitstreams/b1647116-b54f-4025-a2f8-6cd5f591c758/download File MD5 0333bcf09fbe98474c3d1b146f944ce1 3161426 application/pdf Lorente-Leal et al 2021.pdf