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Docta Complutense author Morales Menchén, Ana author Navarro García, Federico author Guirao Abad, José P. author Román González, Elvira author Prieto Prieto, Antonio Daniel author Coman, Ioana V author Pla Alonso, Jesús author Alonso Monge, Rebeca María Del Mar 2023-06-17T13:23:57Z 2023-06-17T13:23:57Z 2018-05-03 Morales Menchén, A., Navarro García, F., Guirao Abad, J. P. et al. «Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii». Frontiers in Cellular and Infection Microbiology, vol. 8, mayo de 2018, p. 135. DOI.org (Crossref), https://doi.org/10.3389/fcimb.2018.00135. 2235-2988 10.3389/fcimb.2018.00135 https://hdl.handle.net/20.500.14352/13366 https//doi.org/10.3389/fcimb.2018.00135 Certain yeasts secrete peptides known as killer toxins or mycocins with a deleterious effect on sensitive yeasts or filamentous fungi, a common phenomenon in environmental species. In a recent work, different () strains isolated from a wide variety of cheeses were identified as producing killer toxins active against and . We have analyzed the killer activity of these toxins in mutants defective in MAPK signaling pathways and found that the lack of the MAPK Hog1 (but not Cek1 or Mkc1) renders cells hypersensitive to mycocins while mutants lacking other upstream elements of the pathway behave as the wild type strain. Point mutations in the phosphorylation site (T174A-176F) or in the kinase domain (K52R) of gene showed that both activities were relevant for the survival of to killer toxins. Moreover, Hog1 phosphorylation was also required to sense and adapt to osmotic and oxidative stress while the kinase activity was somehow dispensable. Although the addition of supernatant from the killer toxin- producing 242 strain (-242) induced a slight intracellular increase in Reactive Oxygen Species (ROS), overexpression of cytosolic catalase did not protect against this mycocin. This supernatant induced an increase in intracellular glycerol concentration suggesting that this toxin triggers an osmotic stress. We also provide evidence of a correlation between sensitivity to -242 killer toxin and resistance to Congo red, suggesting cell wall specific alterations in sensitive strains. eng Atribución 3.0 España Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii journal article URL https://docta.ucm.es/bitstreams/bcd14af4-15b0-44fe-b1db-209baf0dc1f7/download File MD5 8330366b1dd29b5ef1de94b40e3c026c 1799978 application/pdf MORALES-MENCHEN A 2018.pdf URL https://docta.ucm.es/bitstreams/60dd1dee-85da-4857-8141-00f1b0b081fd/download File MD5 1268364e509e61895f23eefb52361aca 76446 text/plain MORALES-MENCHEN A 2018.pdf.txt