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Docta Complutense author Vaz, Catarina Oliveira author Reales Calderón, José Antonio author Pitarch Velasco, Aída author Vellosillo, Perceval author Trevisan Herraz, Marco author Hernáez Sánchez, Maria Luisa author Monteoliva Díaz, Lucía author Gil , Concha 2023-06-17T13:24:41Z 2023-06-17T13:24:41Z 2019-04-15 Vaz, C. O., Reales Calderón, J. A., Pitarch Velasco, A. et al. «Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida Albicans». Journal of Proteome Research, vol. 18, n.o 5, mayo de 2019, pp. 2139-59. DOI.org (Crossref), https://doi.org/10.1021/acs.jproteome.9b00032. 1535-3893 10.1021/acs.jproteome.9b00032 https://hdl.handle.net/20.500.14352/13404 https//doi.org/10.1021/acs.jproteome.9b00032 https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00032 Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways are activated, leading to the production of cytokines, chemokines, and antimicrobial peptides. ATP binding proteins are crucial for this regulation. Here, a quantitative proteomic and phosphoproteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. From a total of 547 nonredundant quantified proteins, 137 were ATP binding proteins and 59 were detected as differentially abundant. From the differentially abundant ATP-binding proteins, 6 were kinases (MAP2K2, SYK, STK3, MAP3K2, NDKA, and SRPK1), most of them involved in signaling pathways. Furthermore, 85 phosphopeptides were quantified. Macrophage proteomic alterations including an increase of protein synthesis with a consistent decrease in proteolysis were observed. Besides, macrophages showed changes in proteins of endosomal trafficking together with mitochondrial proteins, including some involved in the response to oxidative stress. Regarding cell death mechanisms, an increase of antiapoptotic over pro-apoptotic signals is suggested. Furthermore, a high pro-inflammatory response was detected, together with no upregulation of key mi-RNAs involved in the negative feedback of this response. These findings illustrate a strategy to deepen the knowledge of the complex interactions between the host and the clinically important pathogen C. albicans. eng Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans journal article URL https://docta.ucm.es/bitstreams/54617deb-d756-42f5-b597-7bba60087bee/download File MD5 83089bb065ce926cc5756214c6dd1113 3112025 application/pdf Vaz et al 2018.pdf URL https://docta.ucm.es/bitstreams/1ebad164-2e1b-44df-85c8-6a0e01d1c8f2/download File MD5 7c4fc72c12dd2ad0c04394572e423f55 126378 text/plain Vaz et al 2018.pdf.txt