2026-06-29T09:30:12Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1340912026-03-19T00:51:16Zcom_20.500.14352_14col_20.500.14352_16

Activation kinetics of regulatory molecules during immunological synapse in T cells Gómez Morón, Álvaro Requena, Silvia Roda Navarro, Pedro Martín Cófreces, Noa B. Roda Navarro, Pedro 612.017 Actin CD3 Cytoskeleton Dynamics Phospholipase C Signaling Synapse T cell Tubulin Ciencias Biomédicas Inmunología 24 Ciencias de la Vida 2412 Inmunología T cell activation through TCR stimulation leads to the formation of the immunological synapse (IS), a specialized adhesion organized between T lymphocytes and antigen presenting cells (APCs) in which a dynamic interaction among signaling molecules, the cytoskeleton and intracellular organelles achieves proper antigen-mediated stimulation and effector function. The kinetics of molecular reactions at the IS is essential to determine the quality of the response to the antigen stimulation. Herein, we describe methods based on biochemistry, flow cytometry and imaging in live and fixed cells to study the activation state and dynamics of regulatory molecules at the IS in the Jurkat T cell line CH7C17 and primary human and mouse CD4+ T lymphocytes stimulated by antigen presented by Raji and HOM2 B cell lines and human and mouse dendritic cells. Agencia Estatal de Investigación (España) Depto. de Inmunología, Oftalmología y ORL Fac. de Medicina TRUE pub 2026-03-18T08:14:08Z 2026-03-18T08:14:08Z 2023-02-27 journal article AM https://hdl.handle.net/20.500.14352/134091 0065-1281 10.1016/bs.mcb.2022.10.014 eng PID2023-147805NB-I00 Alvaro Gomez-Moron et al. Chapter 10 - Activation kinetics of regulatory molecules during immunological synapse in T cells. Methods in Cell Biology Volume 178, 2023, Pages 149-171 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf Elsevier