2026-06-29T03:13:08Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1349112026-04-22T00:05:37Zcom_20.500.14352_14col_20.500.14352_20
Docta Complutense author López Vázquez, Ana author Torrado Maya, Alejandro author Hervás, Manuel author Navarro, José. A. author Reyes Sosa, Francisco M. author Díez, Bruno author Molina Heredia, Fernando P. 2026-04-21T08:41:48Z 2026-04-21T08:41:48Z 2016-05-25 Ana López Vázquez, Alejandro Torrado, Manuel Hervás, José A. Navarro, Francisco M. Reyes-Sosa, Bruno Díez, Fernando P. Molina-Heredia, Improving enzyme cocktails for lignocellulose hydrolysis in biorefineries by rational protein design, New Biotechnology, Volume 33, Issue 3, 2016, Page 410, ISSN 1871-6784, https://doi.org/10.1016/j.nbt.2015.10.067. (https://www.sciencedirect.com/science/article/pii/S1871678415002368) 1871-6784 10.1016/j.nbt.2015.10.067 https://hdl.handle.net/20.500.14352/134911 1876-4347 10.1016/j.nbt.2015.10.067 https://www.sciencedirect.com/science/article/pii/S1871678415002368 Plant biomass provides an abundant source of sustainable energy and chemical building blocks, mainly in the form of carbohydrates, which can be used in the newly established biorefineries through the release of fermentable sugars. Fermentation of these sugars can produce valuable commercial end products such as biofuels (i.e. bioethanol) and biochemicals. The improvement of enzymatic reactions to hydrolyze biomass to fermentable sugars is essential to improve the profitability of the process. An area of research directed at reducing costs and improving the yield of biofuel production processes is focused on improving the technical efficiency of the individual enzymes, or of the whole enzyme cocktails used to generate fermentable sugars from biomass. Using protein-engineering techniques, we have designed structural and functional mutants of different cellulolytic enzymes. Rational mutants have been designed through evolutionary trace studies, based on similar functions and relevant characteristics to acquire enhanced stability in specific culture conditions. This process is becoming a powerful tool for the rational design of engineered enzymes. eng Improving enzyme cocktails for lignocellulose hydrolysis in biorefineries by rational protein design conference output 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URL https://docta.ucm.es/bitstreams/697ea982-2c40-41dc-ab95-b080872e0a85/download File MD5 cd85b6dc4354b83f630f9cb943f7ca28 52359 application/pdf Improving_enzyme_cocktails.pdf