2026-06-29T02:42:26Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1350252026-04-25T00:06:51Zcom_20.500.14352_14col_20.500.14352_15

Divergent Macroscopic and Microscopic Patterns in Bony‐Fibrous Entheses Revealed by a Multidisciplinary Approach DAngelo del Campo, Manuel Domingo Fiorentino, Claudia López‐Rey, José María Cambra Moo, Óscar Martín, Armando González García Martínez, Daniel Salega, Soledad This work was supported by the Ministerio de Ciencia, Innovación y Universidades, Reino de España (PGC2018-099405-B-I00), the Ministerio de Economía y Competitividad, Reino de España (HAR2016-78036-P), the Consejo Nacional de Investigaciones Científicas y Técnicas, the Comunidad de Madrid (SI4/PJI/2024-00104), and the FONCyT, Ministerio de Ciencia, Tecnología e Innovación, República Argentina (PICT 2021-00815). Introduction. Entheseal changes are influenced by factors such as physical activity, age, sex, and genetics. While macroscopic and 3D geometric morphometric approaches have been used to study EC, the relationship between macroscopic appearance and microstructure remains still poorly understood. Material and Methods. M. deltoideus enthesis was analyzed in nine adult male humeri from Santa María de la Soledad ossuary in Almansa (12th–18th centuries, Spain). Macroscopic robusticity was recorded using Mariotti method to evaluate entheseal changes, while microanatomical analysis combined m-CT scanning and histological thin sections. Mineralized area (% Min. Ar.) was quantified using Fiji-ImageJ, and spatial distribution of non-mineralized cavities was mapped using GIS software. A Kruskal-Wallis test was performed in RStudio. Results. The value and distribution of the percentage of mineralized area (% Min. Ar.) depend on the degree of robusticity. Grade 3 entheses showed lower and more variable mineralization, with a valley-shaped distribution. Grades 1 and 2 entheses had a flat-distributed % Min. Ar. whose mean was over 90%. Histological analysis revealed secondary osteon dominance, while spatial analysis showed concentration of bone cavities within the entheses development areas. Conclusion. The study suggests that macroscopic EC grades may not represent successive phases of bone remodeling and adaptation to mechanical loading. Bone growth of entheses with degrees 1 and 2 is gradual, with a compact and well-organized internal anatomy, whereas entheses with degree 3 may result from extreme mechanical stress that triggers intense bone (re)modeling. Micro-CT and histology provided complementary insights, supporting non-destructive methods for future research. 2026-04-24T08:50:37Z 2026-04-24T08:50:37Z 2026-01-30 journal article DAngelo del Campo, M. D., Fiorentino, C., López-Rey, J. M., Cambra-Moo, Ó., Martín, A. G., García-Martínez, D., & Salega, S. (2026). Divergent Macroscopic and Microscopic Patterns in Bony-Fibrous Entheses Revealed by a Multidisciplinary Approach. American Journal of Biological Anthropology, 189(2). https://doi.org/10.1002/AJPA.70207 2692-7691 10.1002/ajpa.70207 https://hdl.handle.net/20.500.14352/135025 1096-8644 https://doi.org/10.1002/ajpa.70207Digital Object Identifier (DOI) https://onlinelibrary.wiley.com/doi/10.1002/ajpa.70207 eng info:eu-repo/grantAgreement/MICIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099405-B-I00G/CONSTRUYENDO LAS IMAGENES DE GUERREROS EN EUROPA. EL PAPEL DEL CAMPANIFORME IBERICO TIPO CIEMPOZUELOS info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/HAR2016-78036-P/FORMAS DE OCUPACION Y ORGANIZACION DEL ESPACIO EN EL NORTE PENINSULAR: EL TERRITORIO ASTUR ENTRE EPOCA ANTIGUA Y MEDIEVAL A TRAVES DEL REGISTRO ARQUEOLOGICO Y PALEOAMBIENTAL S2024/SI4/PJI/2024-00104/IMPLEMENTACIÓN DE TÉCNICAS DE ANTROPOLOGÍA VIRTUAL PARA EL ESTUDIO DE COLECCIONES OSTEOLÓGICAS HUMANAS DE LA UNIVERSIDAD AUTÓNOMA DE MADRID: ANÁLISIS DE LA VARIACIÓN EN LOS VOLÚMENES DEL HUESO CORTICAL Y LA DENTINA EN SERES HUMANOS ANATÓMICAMENTE MODERNOS restricted access John Wiley & Sons